Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labraxL.) reared in freshwater and marine facilities in Greece

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Publiée : Nov 29, 2017
DOI : https://doi.org/10.12681/jhvms.15074
E. XYLOURI (Ε. ΞΥΛΟΥΡΗ)
Department of Anatomy & Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens
J. KOTZAMANIS (Ι. ΚΟΤΖΑΜΑΝΗΣ)
Institut für Physiologische Chemie und Pathobiochemie, Abteilung Angewandte Molekularbiologie Johannes Gutenberg-Universität; Laboratory of Fish Nutrition and Pathology, Institute of Aquaculture, Hellenic Centre for Marine Research
F. ATHANASSOPOULOU (ΑΘΑΝΑΣΟΠΟΥΛΟΥ Φ.)
Laboratory of Ichthyology, Fish Diseases & Aquaculture, Faculty of Veterinary Medicine; School of Health Sciences, University of Thessaly
L. DONG
Department of Anatomy & Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens
A. ARGYROKASTRITIS (ΑΛ. ΑΡΓΥΡΟΚΑΣΤΡΙΤΗΣ)
Department of Anatomy & Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens
E. FRAGKIADAKI (ΕΙΡ. ΦΡΑΓΚΙΑΔΑΚΗ)
Department of Anatomy & Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens
Résumé

The present study aims το characterize Noda virus isolates from freshwater and marine fish, specifically from species Dicentrarchus labrax (sea bass). Previous works reported the detection of Noda virus only by using PCR, in cultured Dicentrarchus labrax showing clinical symptoms. Here we describe the isolation of Noda virus from symptomatic sea bass cultured in sea and freshwater facilities in Greece. The virus was isolated in the continuous cell line SSN -1, exhibiting characteristic cytopathic effect, vacuolation and degeneration of the monolayer. In parallel, in all cerebral tissue samples isolated from infected individuals, a 255 bp viral fragment has been detected, using Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and nested PCR. Comparison of the amplified sequence was detected in diseased fish in European farms and in other piscine species revealed a high nucleotide homology.

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