Sensitivity of preputial and vaginal exfoliative cytological examination for diagnosis of canine leishmaniosis (Leishmania infantum)

PDF (English)
Publiée : Dec 20, 2017
DOI : https://doi.org/10.12681/jhvms.15510
L. V. ATHANASIOU (Λ.Β. ΑΘΑΝΑΣΙΟΥ)
Faculty of Veterinary Medicine, University of Thessaly
M. K. CHATZIS (Μ.Κ. ΧΑΤΖΗΣ)
Faculty of Veterinary Medicine, University of Thessaly
P. G. GOULETSOU (Π.Γ. ΓKΟΥΛΕΤΣΟΥ)
Faculty of Veterinary Medicine, University of Thessaly
M. N. SARIDOMICHELAKIS (Μ.Ν. ΣΑΡΙΔΟΜΙΧΕΛΑΚΗΣ)
Faculty of Veterinary Medicine, University of Thessaly
Résumé

Aim of the study was to examine sensitivity of preputial and vaginal exfoliative cytological examination as a noninvasive alternative to lymph node, spleen and bone marrow cytology, for detection of Leishmania infantum amastigotes in dogs with leishmaniosis, as, in previous studies, the protozoa have been observed in the penis and prepuce of male dogs and in the vagina of female dogs with leishmaniosis. In total, 20 male  and 18 female dogs with confirmed leishmaniosis were included in
the study. Three cytology smears were prepared from different sites of the preputial cavity of males and one smear was prepared from the anterior vagina of females. Leishmania amastigotes were not observed in these samples after microscopic examination for 20 min at 1,000× magnification. Therefore, preputial and vaginal exfoliative cytology is not recommended for routine diagnosis of canine leishmaniosis.

Article Details
  • Rubrique
  • Research Articles

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Références
Baneth G (2010) Canine leishmaniosis: epidemiology, diagnosis and spectrum of infection. Proceedings of the 24th Annual Congress of the E.S.V.D.-E.C.V.D., Florence, Italy, p. 25-30.
Diniz SA, Melo MS, Borges AM, Bueno, R, Reis BP, Tafuri WL, Nascimento EF, Santos RL (2005) Genital lesions associated with visceral leishmaniasis and shedding of Leishmania sp. in the semen of naturally infected dogs. Vet Pathol. 42:650-658.
Feldman EC, Nelson RW (2004) In: (eds Feldman EC, Nelson RW) Canine and Feline Endocrinology and Reproduction. 3rd edn. Saunders, St. Louis, pp. 953-960.
Naucke TJ, Lorentz S (2012) First report of venereal and vertical transmission of canine leishmaniosis from naturally infected dogs in Germany. Parasit Vectors 5:67.
Saridomichelakis MN (2009) Advances in the pathogenesis of canine leishmaniosis: epidemiologic and diagnostic implications. Vet Dermatol 20:471-489.
Saridomichelakis MN, Mylonakis ME, Leontides LS, Koutinas AF, Billinis C, Kontos VI (2005) Evaluation of lymph node and bone marrow cytology in the diagnosis of canine leishmaniasis (Leishmania infantum) in symptomatic and asymptomatic dogs. Am J Trop Med Hyg 73:82-86.
Silva FL, Oliveira RG, Silva TM, Xavier MN, Nascimento EF, Santos RL (2009) Venereal transmission of canine visceral
leishmaniasis. Vet Parasitol 160:55-59.
Silva FL, Rodrigues AA, Rego IO, Santos RL, Oliveira RG, Silva TM, Xavier MN, Nascimento EF, Santos RL (2008) Genital lesions and distribution of amastigotes in bitches naturally infected with Leishmania chagasi. Vet Parasitol 151: 6-90.
Solano-Gallego L, Morell P, Arboix M, Alberola J, Ferrer L (2001) Prevalence of Leishmania infantum infection in dogs living in an area of canine leishmaniasis endemicity using PCR on several tissues and serology. J Clin Microbiol 39:560-563.
Strauss-Ayali D, Jaffe CL, Burshtain O, Gonen L, Baneth G (2004) Polymerase chain reaction using noninvasively obtained samples, for the detection of Leishmania infantum DNA in dogs. J Infect Dis 189:1729-1733.
Articles les plus lus par le même auteur ou la même autrice