Molecular analysis of Peste des Petits Ruminants Virus from outbreak in Turkey during 2010-2012

PDF (English)
DOI : https://doi.org/10.12681/jhvms.21784
A. SAIT
Pendik Veterinary Control Institute, Department of Virology, Istanbul-Turkey
S.B. DAGALP
Ankara University, Faculty of Veterinary Medicine, Department of Virology, Ankara-Turkey
Résumé

The aim of the study is to determine the epizootiology of Peste des petits ruminants (PPR) in Turkey during 2010-2012, using molecular genotyping. Samples of blood (n=193), swab (n=7) and tissue (n=374) were collected from sheep (n=473) and goats (n=101) suspected of having PPRV infection from an outbreak in 50 provinces of Turkey during 2010–2012. These samples (n=574) were tested using reverse transcription polymerase chain reaction (RT-PCR) and real-time reverse transcription

polymerase chain reaction (RT-qPCR) targeting selected parts of the fusion (F) and the nucleocapsid (N) genes. Positivity ratios were 35.5%, 39.3%, and 44.4% with regards to RT-PCR targeting the F and the N genes, and RT-Qpcr targeting the latter gene (N), respectively. The overall positivity rate was 45.8%. For sequence analyses, F-gene (n=53) and N-gene (n=60) positive samples representing different provinces were selected. After phylogenetic analysis, the circulating PPRV was located in lineage IV according to two gene regions. The F-gene partial sequence analysis at the nucleotide level showed 98.2-100% resemblence among 53 for F-gene, and 97.9-98.9% and 91.3-92.4% to Turkey2000 and Nigeria75/1 sequences, respectively. The N-gene partial sequence analysis at the nucleotide level showed 94.2-100% resemblence among 60 for N-gene, and 94.2-98.3% and 89.3-90.9% to Turkey2000 and Nigeria75/1 sequences, respectively. The result of this study indicates that PPRV infection is enzootic in Turkey, and belongs to the lineage IV, which is present in three haplogroup. The phylogenic analysis indicates the spread of the virus is associated with unauthorized movement of stock.

Article Details
  • Rubrique
  • Research Articles
Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Références
Alcigir G, Atalay VS, Toplu N (1996) Türkiye’de kuzularda peste des petits ruminants virus enfeksiyonunun patomorfolojik ve immunohistolojik ilk tanımı. Ankara Univ Vet Fak Derg 43:181-189 (in Turkish with German abstract).
Anderson J, McKay JA (1994) The detection of antibodies against peste des petitsruminants virus in cattle, sheep and goats and the possible implication to rinderpestcontrol programme. Epidemiolo Infect 112:225–231.
Anees M, Shabbir MZ, Muhammad K, Nazir J, Shabbir MAB, Wensman JJ, Munir M (2013) Genetic analysis of peste des petits ruminants virus from Pakistan. BMC Veterinary Research 9(1):60.
Bailey D, Banyard A, Dash P, Ozkul A, Barrett T (2005) Full genome sequence of peste des petits ruminants virus, a member of the Morbillivirus genus. Virus Res 110:119-124.
Bandelt HJ, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37-48.
Banyard AC, Parida S, Batten C, Oura C, Kwiatek O, Libeau G (2010) Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control. J Gen Virol 91:2885-2897.
Bao J, Li L, Wang Z, Barrett T, Suo L, Zhao W, Li J (2008) Development of one-step real-time RT-PCR assay for detection and quantitation of peste des petits ruminants virus. J Virol Methods 148:232-236.
Batten CA, Banyard AC, King DP, Henstock MR, Edwards L, Sanders A, Barrett T (2011) A real time RT-PCR assay for the specific detection of Peste des petits ruminants virus. J Virol Methods 171:401-404.
Couacy-Hymann E, Roger F, Hurard C, Guillou JP, Libeau G, Diallo A (2002) Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. J Virol Methods 100:17-25.
Ezeibe MCO, Okoroafor ON, Ngene AA, Eze JI, Eze IC, Ugonabo JAC (2008) Persistent detection of peste des petits ruminants antigen in the faeces of recovered goats. Trop Anim Health Prod 40:517-519.
Food and Agriculture Organization (2011) Joint FAO/OIE Committee on Global Rinderpest Eradication FINAL REPORT May 2011. https://www.oie.int/doc/ged/D10943.PDF [accessed October 31, 2018]
Food and Agriculture Organization (2012) Recognizing Peste des Petits Ruminants.A Field Manual. http://www.fao.org/docrep/003/x1703e/x1703e00.htm [accessed September 2, 2012]
Forsyth MA, Barrett T (1995) Evaluation of polymerase chain reaction for thedetection and characterisation of rinderpest and peste des petits ruminantsviruses for epidemiological studies. Virus Res. 39:151-163.
Forster P, Torroni A, Renfrew C, Röhl A (2001) Phylogenetic star contraction applied to Asian and Papuan mtDNA evolution. Mol Biol Evol 18:1864-1881.
Ghosh A, Joshi VD, Shaila MS (1995) Characterization of an in vitro transcription system from rinderpest virus. Vet Microbiol. 44:165-173.
Kerur N, Jhala MK, Joshi CG (2008) Genetic characterization of Indian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments. Res Vet Sci. 85:176-183.
Kul O, Kabakci N, Atmaca HT, Özkul A (2007) Natural peste des petits ruminants virus infection: novel pathologic findings resembling other morbillivirus infections. Vet Pathol. 44:479-486.
Kwiatek O, Minet C, Grillet C, Hurard C, Carlsson E, Karimov B, Libeau G (2007) Peste des petits ruminants (PPR) outbreak in Tajikistan. J Comp Pathol. 136:111-119.
Kwiatek O, Keita D, Gil P, Fernández-Pinero J, Clavero MAJ, Albina E, Libeau G (2010) Quantitative one-step real-time RT-PCR for the fast detection of the four genotypes of PPRV. J Virol Methods. 165:168-177.
Libeau G, Diallo A, Parida S (2014) Evolutionary genetics underlying the spread of peste des petits ruminants virus. Animal Frontiers. 4:14-20.
Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451-1452.
Mahajan S, Agrawal R, Kumar M, Mohan A, Pande N (2014) Comparative evaluation of different F and N gene based reverse transcription polymerase chain reaction for molecular detection of peste des petits ruminants virus from clinical samples. Veterinarski Arhiv 84:485-492.
Munir M, Zohari S, Saeed A, Khan QM, Abubakar M, LeBlanc N, Berg M (2012a) Detection and phylogenetic analysis of peste des petits ruminants virus isolated from outbreaks in Punjab, Pakistan. Transbound Emerg Dis 59:85-93.
Munir M, Zohari S, Suluku R, LeBlanc N, Kanu S, Sankoh FAR, Ståhl K (2012b) Genetic characterization of peste des petits ruminants virus, Sierra Leone. Emerg Infect Dis. 18:193-195.
Ozkul A, Akca Y, Alkan F, Barrett T, Karaoglu T, Dagalp SB, Burgu I (2002) Prevalence, distribution, and host range of Peste des petits ruminants virus, Turkey. Emerg Infect Dis. 8:708-712.
Shaila MS, Shamaki D, Forsyth MA, Diallo A, Goatley L, Kitching RP, Barrett T (1996) Geographic distribution and epidemiology of peste des petits ruminants viruses. Virus Res. 43:149-153.
Tatar N, Alkan F (1999) Koyun ve keçilerde küçük ruminantların vebası (peste des petits ruminants) ve sığır vebası enfeksiyonlarının serolojik ve virolojik olarak araştırılması. Etlik Vet. Mikrob. Derg., 10(2):35-60.
Yesilbag K, Yilmaz Z, Golcu E, Ozkul A (2005) Peste des petits ruminants outbreak in western Turkey. Vet Rec. 157:260-261.
Zhao WH, Yang SB, Han JQ, Jiang M, Li HC, Li QH (2009) Confirmed diagnosis by RT-PCR and phylogenetic analysis of peste des petits ruminants viruses in Tibet, China. Virol. Sin. 24:573-578.
Zhiliang W, Jingyue B, Xiaodong W, Yutian L, Lin L, Chunju L, Longciren V, Zhonglun X, Wenji Z, Wei Z, Nan Y, Jinming L, Shushuang W, Junwei W (2009) Peste des petits ruminants virus in Tibet, China. Emerg Infect Dis. 15:299–301.
Articles les plus lus par le même auteur ou la même autrice