Evaluation of Precision Xceed® for on-site monitoring of blood ß-hydroxybutyric acid and glucose in dairy cows


N. PANOUSIS (Ν. ΠΑΝΟΥΣΗΣ)
M. KRITSEPI (Μ. ΚΡΙΤΣΕΠΗ)
I. KARAGIANNIS (Ι. ΚΑΡΑΓΙΑΝΝΗΣ)
E. KALAITZAKIS (ΕΜ. ΚΑΛΑΪΤΖΑΚΗΣ)
E. LAFI
Ch. BROZOS (Χ. ΜΠΡΟΖΟΣ)
Abstract

The aim of the study was to evaluate the usefulness of the Precision Xceed® hand-held meter as an on-site method for determining blood ß-hydroxyboutyric acid (BHBA) and glucose concentrations, for the diagnosis of subclinical ketosis in dry and lactating dairy cows. A total of 163 clinically healthy Holstein cows (113 lactating, 8-50 days-in-milk; and 50 dry, 10-40 days pre-partum) from 5 farms located around Thessaloniki region, were blood-sampled once, from the jugular vein of each animal, 5 to 8 hours after the start of morning feeding. BHBA was determined in all 163 cows, whereas glucose only in 114 cows (50 dry and 64 lactating cows). These analyses were performed, for each cow, by both laboratory method (in serum) and Precision Xceed® meter (in whole blood, cowside). Using laboratory serum BHBA concentrations > 1.2 mmol/L as the cut-off point, 11/163 (6.7%) of the tested cows were considered as subclinically ketotic, whereas raising the cut-off to > 1.4 mmol/L, 9/163 (5.5%) cows had subclinical ketosis. All these cows (11 and 9, respectively) were lactating. None of the dry cows had subclinical ketosis at BHBA cut-off of > 1.4 mmol/L. One out of the 50 dry cows (2%) and 15/113 (13.3%) lactating cows sampled were classified as subclinically ketotic when the Precision Xceed® meter was set at BHBA concentrations > 1.2 mmol/L. Overall, mean BHBA and glucose concentrations were not statistically different (P>0.05) between the two methods. Significant positive correlations were found for BHBA (strong correlation: r=0.99; n=163; P<0.01) and glucose (moderate correlation: r=0.63; n=114; P<0.01) concentrations between Precision Xceed® and laboratory results. Precision Xceed® is less accurate for measuring glucose (glucometer) compared to BHBA (ketometer). The low percentage of false positive (<0.6%) and false negative (<4%) indicating that the Precision Xceed® meter is an accurate screening test and its results are highly reliable under field conditions. Precision Xceed® meter was highly sensitive (90.9%) and specific (96.05%) at cut off point of BHBA concentrations > 1.2 mmol/L and it had excellent test agreement for detection of subclinical ketosis when using a threshold of blood BHBA > 1.4 mmol/L.

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Riferimenti bibliografici
Barham D, Trinder Ρ (1972) Improved colour reagent for determination of blood glucose by oxidase system. Analyst 97:142-145.
Bruss ML (1997) Lipids and ketones. In: Clinical Biochemistry of Domestic Animals, 5th ed. Kaneko JJ, Harvey JW, Bruss ML (Eds.), Academic Press, San Diego: pp 83-117.
Byrne HA, Tieszen KL, Hollis S, Dornan TL, New JP (2000) Evaluation of an electrochemical sensor for measuring blood ketones. Diabetes Care 23:500-503.
Carrier J, Stewart S, Godden S, Fetrow J, Rapnicki Ρ (2004) Evaluation and use of three cowside tests for detection of subclinical ketosis in early postpartum cows. J Dairy Sci 87:3725-3735.
Chiù RW, Ho CS, Tong SF, Ng KF, Lam CW (2002) Evaluation of a new handheld biosensor for point-of-care testing of whole blood beta-hydroxybutyrate concentration. Hong Kong Med J 8:172-176.
Duffield Τ (2000) Subclinical ketosis in lactating dairy cattle. Vet Clin Ν Am - Food A 16:231-253.
Duffield Τ (2003) Minimizing subclinical metabolic diseases. In: Proceedings of Tri-State Dairy Nutrition Conference, Fort Wayne, IN, USA: pp 1-14.
Eicher R, Liesegang A, Bouchard E, Tremblay A (1998) Influence of concentrate feeding frequency and intrinsic factors on diurnal variations of blood metabolites in dairy cows. In: Proceedings of the 31st Annual Convention of the American Association of Bovine Practitioners, Rome, GA, USA: pp 198-202.
Gau Ν (1987) Acetoacetic acid. In: Methods in Clinical Chemistry. Pesce AJ, Kaplan LA (Eds.), CV Mosby Co., St. Louis, MO: pp 97-104.
Geishauser T, Leslie K, Kelton D, Duffield Τ (1998) Evaluation of five cowside tests for use with milk to detect subclinical ketosis in dairy cows. J Dairy Sci 81:438-443.
Geishauser T, Leslie K, Tenhag J, Bashiri A (2000) Evaluation of eight cow side ketone tests in milk for detection of subclinical ketosis in dairy cows. J Dairy Sci 83:296-299.
Geishauser T, Leslie K, Kelton D, Duffield Τ (2001) Monitoring for subclinical ketosis in dairy herds. Comp Cont Educ Pract Vet 23:S65-S70.
Herdt TH (2000) Ruminant adaptation to negative energy balance: Influences on the etiology of ketosis and fatty liver. Vet Clin Ν Am -Food A 16:215-230.
Hoenig M, Dorfman M, Koenig A (2008) Use of a hand-held meter for the measurement of blood beta-hydroxybutyrate in dogs and cats. J Vet Emerg Crit Car 18:86-87.
Iwersen M, Falkenberg U, Voigtsberger R, Forderung D, Heuwieser W (2009) Evaluation of an electronic cowside test to detect subclinical ketosis in dairy cows. J Dairy Sci 92:2618-2624.
Jeppesen R, Enemark JMD, Enevoldsen C (2006) Ketone body measurement in dairy cows. In: Proceedings of the 24th World Buiatrics Congress, Nice, France: Ref: OS 43-2.
Katsoulos PD, Minas A, Karatzia M, Pourliotis K, Christodoulopoulos G (2011) Evaluation of a portable glucose meter for use in cattle and sheep. Vet Clin Pathol 40:245-247.
Kronfeld DS, Raggi F, Ramberg CF Jr. (1968) Mammary blood flow and ketone metabolism in normal, fasted and ketotic cows. Am J Physiol 215: 218-227.
Merck (2011) Ketosis in Cattle: Introduction. In: Merck Sharp & Dohme Corp., a subsidiary of Merck & Co., Inc., Whitehouse Station, NJ, USA. http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/80900.htm [Accessed 2 May 2011].
Nielen M, Aarts MGA, Jonkers AGM, Wensing T, Schukken YH (1994) Evaluation of two cowside tests for the detection of subclinical ketosis in dairy cows. Can Vet J 35:229-232.
Noyes KJ, Crofton P, Bath LE, Holmes A, Stark L, Oxley CD, Kelnar CJH (2007) Hydroxybutyrate near-patient testing to evaluate a new end-point for intravenous insulin therapy in the treatment of diabetic ketoacidosis in children. Pediatr Diabetes 8:150-156.
Oetzel GR (2004) Monitoring and testing dairy herds for metabolic disease. Vet Clin Ν Am - Food A 20:651-674.
Oetzel GR (2007) Herd-Level Ketosis - Diagnosis and Risk Factors. In: Pre-conference Seminar 7C: Dairy Herd Problem Investigation Strategies: Transition Cow Troubleshooting, 40th Annual Conference of the American Association of Bovine Practitioners, Vancouver, BC, Canada: pp 67-91.
Oetzel GR, McGuirk S (2007) Fact sheet - Cowside blood BHBA testing with a hand-held "ketometer"- version 4,3/5/09, School of Veterinary Medicine, University of Wisconsin-Madison, USA.
Osborne TM, Leslie KE, Duffield T, Petersson CS, Ten Hag J, Okada Y (2002) Evaluation of keto-test in urine and milk for the detection of subclinical ketosis in periparturient Holstein dairy cattle. In: Proceedings of the 35th Conference of the American Association of Bovine Practitioners, Rome, GA, USA: pp 188-189.
Rollin F (2006) Tools for a prompt cowside diagnosis: what can be implemented by the bovine practitioner? In: Proceedings of the 24th World Buiatrics Congress, Nice, France: pp 75-85.
Sakha M, Ameri M, Sharifi H, Taheri I (2007) Bovine subclinical ketosis in dairy herds in Iran. Vet Res Commun 31:673-679.
Tyopponen J, Kauppinen Κ (1980) The stability and automatic determination of ketone bodies in blood samples taken in field conditions. Acta Vet Scand 21:55-61.
Voyvoda H, Erdogan Η (2010) Use of a hand-held meter for detecting subclinical ketosis in dairy cows. Res Vet Sci 89:344-351.
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