Genetic structure and diversity among three Greek sheep breeds using Random Amplified Polymorphic DNA-PCR

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Pubblicato: Nov 13, 2017
DOI: https://doi.org/10.12681/jhvms.14860
I. MASTRANESTASIS (Ι. ΜΑΣΤΡΑΝΕΣΤΑΣΗΣ)
Biodiversity Conservation Laboratory,Department of Environment, University of the Aegean ; National Agriculture Research Foundation-NAGREF
Ch. LIGDA (Χρ. ΛΙΓΔΑ)
National Agriculture Research Foundation- NAGREF, Veterìnary Research Institute of Thessaloniki
K. THEODOROU (Κ. ΘΕΟΔΩΡΟΥ)
Biodiversity Conservation Laboratory,Department of Environment,University of the Aegean
L. V. EKATERINIADOU (Λ.Β. ΑΙΚΑΤΕΡΙΝΙΑΔΟΥ)
National Agriculture Research Foundation- NAGREF
Abstract

Genetic structure and diversity of 120 animals from three Greek local breeds were investigated by Random Amplified Polymorphic DNA (RAPD) - PCR method. Sheep samples originated from the Lesvos, Chios and Karagouniko breeds were treated with 11 random primers to estimate their genetic diversity and phylogenetic relationships. Our analysis comprised two levels of the breeds' genetic structure: i) the genetic differentiation among the three breeds and ii) the genetic differentiation among the flocks within each breed. This combined approach gave two main findings: i) the study of genetic distances and identity revealed that the Karagouniko sheep breed is genetically distinct from Chios (GD=0.1979) and Lesvos (GD=0.1691) breeds, while ii) the Chios and Lesvos breeds are genetically similar (GI=0.9631); half of the flocks of Lesvos have a relatively closer relationship with those of Chios than with the other Lesvos flocks. This is the first study that reports the close genetic relationship between the Chios and Lesvos breeds and gives strong evidence to hypotheses about their related origin. Furthermore, the study of polymorphic loci revealed particular indicators located in Karagouniko breed, as definitional datum of genetic identity or as a fingerprint of breed. Therefore, RAPD-DNA methods can be an efficient tool for the determination of phylogenetic relationships and genetic identity among the bloodstock breed of sheep.

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Riferimenti bibliografici
Abdel-Rahman, S. M., Hafez, Ε. E. (2007) Genetic similarity among the three Egyptian water buffalo flocks using RAPD-PCR and PCR-RFLP techniques. Res. J. of Agri, and Biol. Sci. 3(5): 351-355.
Ali, B.A. (2003) Genetic similarity among four breeds of sheep in Egypt detected by random amplified polymorphic DNA markers. Afr. J. Biotech. 2:194-197.
Appa Rao, K.B.C., Bhat, K.V., Totey, S.M. (1996) Detection of species - specific genetic markers in farm animals through random amplified polymorphic DNA (RAPD). Genet. Anal.: Biomol. Eng. 13:135-138.
Benieri, D. (2005) Determination of human or not origin of coliform that is isolated by the aquatic environment with cultures and molecular techniques. Doctoral thesis. Department of Medicine. University of Patras (in Greek): 33.
Benter, T., Papadopoulos, S., Pape, M., Manns, M. and Poliwoda, H. (1995) Optimization and reproducibility of Random Amplified Polymorphic DNA in human. Anal Biochem. 230: 92-100.
Bhattacharya, T. K., Kumar, P., Joshi, J. D., Kumar, S. (2003) Estimation of inbreeding in cattle using RAPD markers. Journal of Dairy Research. 70:127-129.
Budak, F. Α., Çakir Α. §. (2009) Molecular Genetic Analysis of Three Turkish Sheep Breeds by RAPD-PCR Method. Int. J. Nat. & Engin. Sci. 3(3): 51-56.
Chattopadhyay, P. (2003) RAPD markers system as a useful tool for rapid identification of the origin of lizard contaminants in food. Food Chem. Toxicol. 41:1719-1725.
Cushwa, W.T., Medrano, J.F. (1994) Identification of RAPD genetic markers in sheep. Proc. 5th. World Congress Genet. Appi. Livestock Prod. 21:133-136.
Directorate of Inputs to Animal Production (2009) Ministry of Rural Development and Food of the Hellenic Republic, http://www.minagric.gr/greek/2.8_zwa_anaparagwgis_menu.html.
Devrim, A. K., Kaya, N., Guven, Α., Kocamis, H. (2007) A study of genomic polymorphism and diversity in sheep breeds in northeastern Anatolia. 73: 291-295.
Dimitriadis, I. (1957) Sheep and Goat Farming. Gartaganis Editions. Thessaloniki: pp 137 (in Greek).
Elmaci, C, Oner, Y., Ozis, S. And Tuncel, E. (2007) RAPD analysis of DNA polymorphism in Turkish sheep breeds. Biochem. Genet. 45:691-696.
FAO (2007) The State of the World's Animal Genetic Resources for Food and Agriculture, edited by Rischkowsky and Pilling. Rome. Georgoudis, Α., Hatziminaoglou, J., Pappas, V. (1995) The breeding scheme of the Karagouniko sheep in Greece. Options Méditerranéennes, Serie A. 11: 61-65.
Handley L., L. J., Byrne, K., Santucci, F., Townsend, S., Taylor, M., Bruford, M. W., Hewitt, G. M. (2007) Genetic structure of European sheep breeds. Heredity. 99: 620-631.
Hatziminaoglou, I. (2001) Sheep and Goats in Greece and world wide. Giahoudi-Giapouli Editions. Thessaloniki: pp. 240
Hatziminaoglou, I., Georgoudis, Α., Zervas, N., Boyazoglu, J. (1996) Prolific Breeds of Greece. In Prolific Sheep. (M.H. Fahmy, ed.), CAB International, University Press, Cambridge, Chap 3.3: pp 542.
Hatziolos, B. (1941) The issue of animal production in Greece. Greek Publishing Company. Athens: pp. 576 (in Greek).
Hlophe S.R. (2011) Genetic variation between and within six selected south African sheep breeds using Random Amplified Polymorphic DNA and protein markers. MSc Thesis.
Karantounias, A. (1964) Estimative of Farm Animals. II. Estimative of Sheep. Ministry of Agriculture, National Press. Athens (in Greek).
Katsaounis, N. (1996) Sheep farming. Kiriakidis Editions. Thessaloniki - Athens: 106-129 (in Greek).
Khaldi, Z., Rekik, B., Haddad, B., Zourgui, L., Souid, S. (2010) Genetic characterization of three ovine breeds in Tunisia using randomly amplified polymorphic DNA markers. Livestock Research forRural Development. 22 (3).
Kimura, M., Crow, J. F. (1964) The number of alleles that can be maintained in a finite population. Genetics. 49: 725-738.
Kumar, K. G., Kumar, P., Kumar, S., Bhattacharya, T. K., Bhusan, B. (2004) Random amplified polymorphic DNA (RAPD) fingerprinting of Indian sheep breeds. Indian J. Anim. Sci. 74: 860-863.
Kumar, S., Kolte, A. P., Yadav, B. R., Kumar, Sus., Arora A. L., Singh, V. K. (2008) Genetic variability among sheep breeds by random amplified polymorphic DNA-PCR. Indian J. Biotechnol. 7: 482-486.
Kunene, N. W., Bezuidenhout, C C, Nsahlai, I. V. (2009) Genetic and phenotypic diversity in Zulu sheep population: Implication for exploitation and conservation. Small Rumin. Res. 84:100-107.
Ligda, Ch., Altarayrah, J., Georgoudis, Α., ECONOGENE Consortium (2009) Genetic analysis of Greek sheep breeds using microsatellite markers for setting conservation priorities. Small Rumin. Res. 83:42-48.
Meunier, J. R. and Grimont, P. A. D. (1993) Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res. Microbiol. 144: 373-379.
Ministry of Rural Development and Food (2004) Results of the Milk Recording in Lesvos breed. Publication of the Animal Genetic Improvement Centre of Athens and Rural Cooperative of Agras. Athens, (in Greek).
Nei, M. (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89:583-590.
Nei, M. (1987) Molecular evolutionary genetics. Columbia University Press. New York, USA
Okumus, A. and Mercan, L. (2007) Genetic variation at Karayaka sheep herbs based on Random Amplified Polymorphic DNA markers. Biotechnology. 6(4): 543-548.
Paiva, S. R., Silvério, V. C, Egito, Α. Α., McManus, C. Faria, D. Α., Mariante, A. S., Castro, S. R., Albuquerque, M. S. M. and Dergam, J. A. (2005) Genetic variability of the Brezilian hair sheep breeds. Pesq. Agropec. Bras., Brasilia. 40: 887-893.
Papavasiliou, D., Bizelis, I., Laskaridis, G., Rogdakis E. (1998) Morphological, reproductive and productive characteristics of Lesvos sheep breed. J Hellenic Soc. of Anim. Prod. Anim. Sci. Rev. (in Greek). 25: 29-43.
Radu, S., Wai Ling, O., Rusul., G., Karim, M.I.A. and Nishibuchi, M. (2001) Detection of Escherichia coli 0157:H7 by multiplex PCR and their characterization by plasmid profiling, antimicrobial resistance, RAPD and PFGE analysis. J. Microbiol Methods. 46:131-139.
Rahman, M.A., Rahman, S.M.M., Jalil, M.A., Uddin, S.N. and Rahman, M.M. (2006) Molecular characterization of Black Bengaland Jamuna Pari goat breeds by RAPD markers. American J.Anim. & Vet. Sci. 1(2): 17-22.
Rogdakis, Ε. (2002) Indigenous sheep breeds: Description, Phylogeny, Genetic Improvement, Conservation. Agrotypos. Athens: pp. 208.
Soûle, M.E., Mills, L.S. (1998) No need to isolate genetics. Science. 282:1658-1659.
Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A.,Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res.18: 6531-6535.
Yeh, F. C, Yang, R. C, Boyle, T. B. J. (1999) POPGENE Version 1.31. Microsoft Window-based Freeware for Population Genetic Analysis. Quick User Guide. University of Alberta and Centre for International Forestry Research. Canada.
Zigogianis, D. (1999) Sheep production. Production systems of ruminants (number A). Current Education. Thessaloniki: 271-283 (in Greek).
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