Effects of Dietary Prebiotic, Probiotic, and Synbiotic Supplementation on Growth Performance, Carcass Traits, Digestive Enzymes and Some Biochemical Parameters in Broiler Chickens


M Olmez
https://orcid.org/0000-0002-5003-3383
T Şahin
Ö Karadağoğlu
M Öğün
S Adıgüzel Işık
B Boğa Kuru
MA Yörük
Abstract

This study was conducted to determine and compare the effects of chitosan-oligosaccharide (COS), probiotics (Pro), and their combination on the growth performance, carcass parameters, digestive enzymes, and serum biochemical parameters of broiler chickens. A total of 300 one-day-old mixed-sex Ross 308 broiler chicks were randomly divided into four treatment groups with five replicates each (15 chicks per replicate). No supplements were added to the basal diet in the control group; however, 1 g/kg COS, 1 g/kg Pro, and 1 g/kg COS + 1 g/kg Pro (Syn) were added to the basal diet of the respective treatment groups. At the end of the study, the results showed that the dietary supplementation of COS, Pro, and Syn significantly increased the average daily gain (P < 0.001) and average feed intake (P < 0.001) in all the treatment groups compared to the control group. COS supplemented group showed the lowest feed conversion ratio (P = 0.004) among all the study groups. There was no significant difference between the groups for carcass parameters and internal organ weights (P > 0.05), except for live weight at slaughter, which was found to be higher in all dietary-supplemented treatment groups than in the control group. There was no significant effect (P > 0.05) of the different diets on digestive enzymes and serum biochemical parameters among the treatment groups (P > 0.05). In conclusion, dietary supplementation of COS and Pro individually and in combination with broiler diets improves performance parameters without adverse effects on serum metabolites and digestive enzymes.

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Riferimenti bibliografici
Abd El Latif MA, Omar MO (2023) Productive performance, digestibility,
blood parameters and intestine microbiology of broiler chicks
affected by prebiotic, probiotic and synbiotic addition. Egypt Poult
Sci 43:217-237.
Ahmad S, Yousaf MS, Tahir SK, Rashid MA, Majeed KA, Naseem M,
Raza M, Hayat Z, Khalid A, Zaneb H (2023) Effects of co-supplementation
of β-galacto-oligosaccharides and methionine on breast
meat quality, meat oxidative stability and selected meat quality genes
in broilers. Pak Vet J 43:428-434.
Al-Khalaifa H, Al-Nasser A, Al-Surayee T, Al-Kandari S, Al-Enzi N, Al-
Sharrah T, Ragheb G, Al-Qalaf S, Mohammed A (2019) Effect of
dietary probiotics and prebiotics on the performance of broiler chickens.
Poult Sci 98:4465-4479.
Aslam MA, İpek E, Riaz R, Ӧzsoy ŞY, Shahzad W, Güleş Ӧ (2021) Exposure
of broiler chickens to chronic heat stress increases the severity
of white striping on the pectoralis major muscle. Trop Anim Health
Prod 53:1-10.
Awad W, Ghareeb K, Abdel-Raheem S, Böhm J (2009) Effects of dietary
inclusion of probiotic and synbiotic on growth performance, organ
weights, and intestinal histomorphology of broiler chickens. Poult Sci
:49-56.
Bilal M, Si W, Barbe F, Chevaux E, Sienkiewicz O, Zhao X (2021) Effects
of novel probiotic strains of Bacillus pumilus and Bacillus subtilis on
production, gut health, and immunity of broiler chickens raised under suboptimal conditions. Poult Sci 100:100871.
Besharati M, Giannenas I, Palangi V, Ayașan T, Maggiolino A, Lorenzo
JM (2022) Chitosan/calcium-alginate encapsulated flaxseed oil on
dairy cattle diet: in vitro fermentation and fatty acid biohydrogenation.
Animals 12:1400
Chang Q, Lu Y, Lan R (2020) Chitosan oligosaccharide as an effective
feed additive to maintain growth performance, meat quality, muscle
glycolytic metabolism, and oxidative status in yellow-feather broilers
under heat stress. Poult Sci 99:4824-4831.
Cheng Y, Chen Y, Li X, Yang W, Wen C, Kang Y, Wang A, Zhou Y (2017)
Effects of synbiotic supplementation on growth performance, carcass
characteristics, meat quality and muscular antioxidant capacity and
mineral contents in broilers. J Food Sci 97:3699-3705.
Danilova I, Sharipova M (2020) The practical potential of bacilli and their
enzymes for industrial production. Front Microbiol 11:1782.
Davani-Davari D, Negahdaripour M, Karimzadeh I, Seifan M, Mohkam
M, Masoumi SJ, Berenjian A, Ghasemi Y (2019) Prebiotics: definition,
types, sources, mechanisms, and clinical applications. Foods
:92.
Egorov I, Egorova T, Yildirim E, Kalitkina K, Ilina L, Frolov V (2022)
Effect of chitosan complexes on the bacterial community of cecum
and productivity of broiler chickens. BIO Web of Conf 48:03007.
Erdoğan Z, Erdoğan S, Aslantaş Ö, Çelik S (2010) Effects of dietary supplementation
of synbiotics and phytobiotics on performance, caecal
coliform population and some oxidant/antioxidant parameters of
broilers. J Anim Physiol Anim Nutr 94:e40-e48.
FAO (2016). Probiotics in animal nutrition: production, impact and regulation.
FAO Animal Production and Health Paper 179.
Gadde U, Kim W, Oh S, Lillehoj HS (2017) Alternatives to antibiotics
for maximizing growth performance and feed efficiency in poultry: a
review. Anim Health Res Rev 18:26-45.
Ghasemi HA, Shivazad M, Mirzapour Rezaei S, Karimi Torshizi M
(2016) Effect of synbiotic supplementation and dietary fat sources on
broiler performance, serum lipids, muscle fatty acid profile and meat
quality. Br Poult Sci 57:71-83.
Ghasemi HA, Taherpour K (2013) Comparative effects of probiotic, prebiotic
and synbiotic supplements on performance, jejunal morphology,
serum lipid profile and antibody response of broiler chicks. J Livest
Sci Technol 1:20-27.
Ghasemi HA, Kasani N, Taherpour K (2014) Effects of black cumin seed
(Nigella sativa L.), a probiotic, a prebiotic and a synbiotic on growth
performance, immune response and blood characteristics of male
broilers. Livest Sci 164:128-134.
Gong L, Wang B, Mei X, Xu H, Qin Y, Li W, Zhou Y (2018) Effects of
three probiotic Bacillus on growth performance, digestive enzyme
activities, antioxidative capacity, serum immunity, and biochemical
parameters in broilers. Anim Sci J 89:1561-1571.
Jabeen Z, Bukhari SA, Malik SA, Hussain G, Kamal S (2023) Improved
gut microbiota escalates muscle function rehabilitation and ameliorates
oxidative stress following mechanically induced peripheral
nerve injury in mice. Pak Vet J 43:707-713.
Iqbal J, Sharif M, Suleman MN, Saeed M, Ahamd F, Kamboh AA, Ayaşan
T, Arslan M (2021) Effect of dietary supplementation of a non-antibiotic
growth promoter on growth performance and intestinal histomorphology
in broilers. Pak J Zool 54:1629-1636.
Hussain K, Abbas A, Alanazi HAH, Alharbi AMA, Alaiiri AA, Rehman A,
Waqas MU, Raza MA, Yasin R, Ahmad B (2023) Immunomodulatory
effects of Artemisia brevifolia extract against experimentally induced
coccidiosis in broiler chicken. Pak Vet J 43:333-338.
Kolodziejski PA, Sassek M, Chalupka D, Leciejewska N, Nogowski L,
Mackowiak P, Jozefiak D, Stadnicka K, Siwek M, Bednarczyk M
(2018) GLP1 and GIP are involved in the action of synbiotics in broiler
chickens. J Anim Sci Biotechnol 9:1-9.
Lan R, Wu F, Wang Y, Lin Z, Wang H, Zhang J, Zhao Z (2024) Chitosan
oligosaccharide improves intestinal function by promoting intestinal
development, alleviating intestinal inflammatory response, and
enhancing antioxidant capacity in broilers aged d 1 to 14. Poult Sci
:103381.
Latorre JD, Hernandez-Velasco X, Wolfenden RE, Vicente JL, Wolfenden
AD, Menconi A, Bielke LR, Hargis BM, Tellez G (2016) Evaluation
and selection of Bacillus species based on enzyme production,
antimicrobial activity, and biofilm synthesis as direct-fed microbial
candidates for poultry. Front Vet Sci 3:95.
Li J, Cheng Y, Chen Y, Qu H, Zhao Y, Wen C, Zhou Y (2019) Dietary chitooligosaccharide
inclusion as an alternative to antibiotics improves
intestinal morphology, barrier function, antioxidant capacity, and immunity
of broilers at early age. Animals 9:493.
Li J, Cheng Y, Chen Y, Qu H, Zhao Y, Wen C, Zhou Y (2019) Effects
of dietary synbiotic supplementation on growth performance, lipid
metabolism, antioxidant status, and meat quality in Partridge shank
chickens. J Appl Anim Res 47:586-590.
Li X, Ding X, Peng X, Chi X, Cui H, Zuo Z, Fang J (2017) Effect of
chitosan oligosaccharides on antioxidant function, lymphocyte cycle
and apoptosis in ileum mucosa of broiler. Kafkas Univ Vet Fak Derg
:571-577.
Li X, Piao X, Kim S, Liu P, Wang L, Shen Y, Jung S, Lee H (2007) Effects
of chito-oligosaccharide supplementation on performance, nutrient
digestibility, and serum composition in broiler chickens. Poult Sci
:1107-1114.
Li Y, Chang W, Zhang S, Zheng A, Liu G, Cai H, Liu W (2016) Effects of
dietary chitosan oligosaccharide and Lactobacillus casei on growth
performance, meat quality and antioxidant function of broilers. Chinese
Journal of Animal Nutrition 28:1450-1461.
Manafi M, Hedayati M, Mirzaie S (2018) Probiotic Bacillus species and
Saccharomyces boulardii improve performance, gut histology and
immunity in broiler chickens. S Afr J Anim Sci 48:379-389.
Maqbool B, Ashraf M, Khaliq S, Ashfaq K (2023) Evaluation of potential
effects of locally isolated Streptomyces species as growth promoter in
commercial broilers. Pak Vet J 43:757-763.
Mathivanan R, Selvaraj P, Nanjappan K (2006) Feeding of fermented soybean
meal on broiler performance. Int J Poult Sci 5:868-872.
Min Y, Yang H, Xu Y, Gao Y (2016) Effects of dietary supplementation of
synbiotics on growth performance, intestinal morphology, sIgA content
and antioxidant capacities of broilers. J Anim Physiol Anim Nutr
:1073-1080.
Mohammed A, Jacobs J, Murugesan G, Cheng H (2018) Effect of dietary
synbiotic supplement on behavioral patterns and growth performance
of broiler chickens reared under heat stress. Poult sci 97:1101-1108.
Mookiah S, Sieo CC, Ramasamy K, Abdullah N, Ho YW (2014) Effects
of dietary prebiotics, probiotic and synbiotics on performance, caecal
bacterial populations and caecal fermentation concentrations of broiler
chickens. J Sci Food Agric 94:341-348.
Musa BB, Duan Y, Khawar H, Sun Q, Ren Z, Elsiddig Mohamed MA,
Abbasi IHR, Yang X (2019) Bacillus subtilis B21 and Bacillus licheniformis
B26 improve intestinal health and performance of broiler
chickens with Clostridium perfringens‐induced necrotic enteritis. J
Anim Physiol Anim Nutr 103:1039-1049.
Naveed M, Phil L, Sohail M, Hasnat M, Baig MMFA, Ihsan AU, Shumzaid
M, Kakar MU, Khan TM, Akabar M (2019) Chitosan oligosaccharide
(COS): An overview. Int J Biol Macromol 129:827-843.
Ningsih N, Respati AN, Astuti D, Triswanto T, Purnamayanti L, Yano
AA, Putra RP, Jayanegara A, Ratriyanto A, Irawan A (2023) Efficacy
of Bacillus subtilis to replace in-feed antibiotics of broiler chickens
under necrotic enteritis-challenged experiments: a systematic review
and meta-analysis. Poult Sci 102:102923.
Nisar H, Sharif M, Rahman M, Rehman S, Kamboh A, Saeed M (2021)
Effects of dietary supplementations of synbiotics on growth performance,
carcass characteristics and nutrient digestibility of broiler
chicken. Braz J Poultry Sci 23:1388.
NRC (1994). National research council. Nutrient requirements of poultry.
th rev. ed. National Academy Press, Washington.
Ölmez M, Şahin T, Karadağoğlu Ö, Metin Ö, Yörük Ma, Dalğa S (2022)
Effect of Probiotic Mixture Supplementation to Drinking Water on
the Growth Performance, Carcass Parameters and Serum Biochemical
Parameters in Native Turkish Geese. Kafkas Univ Vet Fak Derg
:131-138.
Ölmez M, Yörük M (2021) Effects of pennyroyal (Mentha pulegium L.)
dietary supplementation on performance, carcass quality, biochemical
parameters and duodenal histomorphology of broilers. J Hellenic
Vet Med Soc 72:3213-3222.
Park YH, Hamidon F, Rajangan C, Soh KP, Gan CY, Lim TS, Abdullah
WNW, Liong MT (2016) Application of probiotics for the production of safe and high-quality poultry meat. Korean J Food Sci Anim Res
:567.
Rashid S, Tahir S, Akhtar T, Altaf S, Ashraf R, Qamar W (2023) Bacillus-
based Probiotics: An Antibiotic Alternative for the Treatment of
Salmonellosis in Poultry. Pak Vet J 43:167-173.
Sahin T, Kaya I, Sari M, Kaya O (2011) The effect of single and combined
use of probiotic and humate in quail (Coturnix coturnix Japonica) diet
on fatttening performance and carcass parameters. Kafkas Univ Vet
Fak Derg 17:1-5.
Sahin T, Kaya I, Unal Y, Elmali DA (2008) Dietary supplementation of
probiotic and prebiotic combination (Combiotics) on performance,
carcass quality and blood parameters in growing quails. J Anim Vet
Adv 7:1370-1373.
Salah AS, El-Tarabany MS, Ali MA (2018) Impact of dietary supplementation
with a synbiotic, organic acids or their combination on growth
performance, carcass traits, economic efficiency, jejunum histomorphometry
and some blood indices of broiler chickens. Anim Prod Sci
:1318-1326.
Salehimanesh A, Mohammadi M Roostaei‐Ali, Mehr M (2016) Effect of
dietary probiotic, prebiotic and synbiotic supplementation on performance,
immune responses, intestinal morphology and bacterial populations
in broilers. J Anim Physiol Anim Nutr 100:694-700.
Sarangi NR, Babu L, Kumar A, Pradhan C, Pati P, Mishra J (2016) Effect
of dietary supplementation of prebiotic, probiotic, and synbiotic on
growth performance and carcass characteristics of broiler chickens.
Vet World 9:313.
Shivaramaiah S, Pumford N, Morgan M, Wolfenden R, Wolfenden A, Torres-
Rodriguez A, Hargis B, Téllez G (2011) Evaluation of Bacillus
species as potential candidates for direct-fed microbials in commercial
poultry. Poult Sci 90:1574-1580.
Sun Y, Zhang Y, Liu M, Li J, Lai W, Geng S, Yuan T, Liu Y, Di Y, Zhang
W (2022) Effects of dietary Bacillus amyloliquefaciens CECT 5940
supplementation on growth performance, antioxidant status, immunity,
and digestive enzyme activity of broilers fed corn-wheat-soybean
meal diets. Poult Sci 101:101585.
Suresh G, Das RK, Kaur Brar S, Rouissi T, Avalos Ramirez A, Chorfi Y,
Godbout S (2018) Alternatives to antibiotics in poultry feed: molecular
perspectives. Crit Rev Microbiol 44:318-335.
Swiatkiewicz S, Arczewska-Wlosek A, Jozefiak D (2014) Feed enzymes,
probiotic, or chitosan can improve the nutritional efficacy of broiler
chicken diets containing a high level of distillers dried grains with
solubles. Livest Sci 163:110-119.
Tarabees R, Gafar KM, El-Sayed MS, Shehata AA, Ahmed M (2019) Effects
of dietary supplementation of probiotic mix and prebiotic on
growth performance, cecal microbiota composition, and protection
against Escherichia coli O78 in broiler chickens. Probiotics Antimicrob
Proteins 11:981-989.
Tayeri V, Seidavi A, Asadpour L, Phillips CJ (2018) A comparison of the
effects of antibiotics, probiotics, synbiotics and prebiotics on the performance
and carcass characteristics of broilers. Vet Res Commun
:195-207.
Teo A-L, Tan H-M (2006) Effect of Bacillus subtilis PB6 (CloSTAT) on
broilers infected with a pathogenic strain of Escherichia coli. J Appl
Poultry Res 15:229-235.
Tufarelli V, Ghavami N, Nosrati M, Rasouli B, Kadim IT, Ramírez LS,
Gorlov I, Slozhenkina M, Mosolov A, Seidavi A, Ayasan T, Laudadio
V (2023) The effects of peppermint (Mentha piperita L.) and chicory
(Cichorium intybus L.) in comparison with a prebiotic on productive
performance, blood constituents, immunity and intestinal microflora
in broiler chickens. 34:3046-3052.
Van Dijk M, Morley T, Rau ML, Saghai Y (2021) A meta-analysis of projected
global food demand and population at risk of hunger for the
period 2010-2050. Nat Food 2:494-501.
Wang J, Ishfaq M, Miao Y, Liu Z, Hao M, Wang C, Wang J, Chen X
(2022) Dietary administration of Bacillus subtilis KC1 improves
growth performance, immune response, heat stress tolerance, and
disease resistance of broiler chickens. Poult Sci 101:101693.
Wang Y, Gu Q (2010) Effect of probiotic on growth performance and digestive
enzyme activity of Arbor Acres broilers. Res Vet Sci 89:163-
Xu Y, Yu Y, Shen Y, Li Q, Lan J, Wu Y, Zhang R, Cao G, Yang C (2021)
Effects of Bacillus subtilis and Bacillus licheniformis on growth performance,
immunity, short chain fatty acid production, antioxidant
capacity, and cecal microflora in broilers. Poult Sci 100:101358.
Yalçın S, Șehu A, Onbașılar E, Șahin T (2003) The effects of dietary humate
and probiotic supplementation on performance of broilers. Ankara
Univ Vet Fak Derg:239-244.
Yazhini P, Visha P, Selvaraj P, Vasanthakumar P, Chandran V (2018) Dietary
encapsulated probiotic effect on broiler serum biochemical parameters.
Vet World 11:1344.
Żbikowski A, Pawłowski K, Śliżewska K, Dolka B, Nerc J, Szeleszczuk
P (2020) Comparative effects of using new multi-strain synbiotics on
chicken growth performance, hematology, serum biochemistry and
immunity. Animals 10:1555.
Zhang S, Zhong G, Shao D, Wang Q, Hu Y, Wu T, Ji C, Shi S (2021)
Dietary supplementation with Bacillus subtilis promotes growth performance
of broilers by altering the dominant microbial community.
Poult Sci 100:100935.
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