Laparoscopic intrauterine insemination in superovulated Chios breed ewes

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Veröffentlicht: Jan 31, 2018
DOI: https://doi.org/10.12681/jhvms.15731
A. LYMBEROPOULOS (Α. ΛΥΜΠΕΡΟΠΟΥΛΟΣ)
N.AG.RE.F., Veterinary Research Institute
B. KÜHHOLZER
Institute of Animal Breeding and Genetics, University of Animal Science, Vienna
G. S. AMIRIDIS (Γ.Σ. ΑΜΟΙΡΙΔΗΣ)
Dept. of Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Thessaly
V. CHRISTODOULOU (Β. ΧΡΙΣΤΟΔΟΥΛΟΥ)
N.AG.RE.F., Animal Research Institute
E. VAINAS (Ε. ΒΑΪΝΑΣ)
N.AG.RE.F., Veterinary Research Institute
Abstract

Superovulation was induced by i.m. administration of 8.8 mg o-FSH in 8 standard doses. Using only one trocar, 0.3 ml of diluted semen (300X106 spermatozoa) was deposited intraluminally, 24-28 hours after oestrus expression, with a 20GX75 mm needle without any fixation of the uterine horns. The duration of the procedure was approximately 5 min per animal. The oocytes-zygotes collection was carried out 18-24 hours after insemination by flushing of the oviducts. The percentage of the collected zygotes varied between donors from 44.44% to 100%. Fertilization was confirmed by the existence of two pronuclei in 121 out of 140 collected oocyteszygotes. Out of the 26 ewes, in 14,10 and 2 the percentage of fertilized oocytes were 100%, 80.55% and 44.44%, respectively. The results from this study are compared with those from other laparoscopic techniques, cervical insemination or natural mating of superovulated ewes. The feasibility for the application of this method in embryo transfer programmes and in intrauterine insemination with frozen ram semen, is also discussed.

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Literaturhinweise
Robinson TJ, Moore NW, Lindsay DR, Fletcher IC, Salamon S. Fertility following synchronisation of oestrus in the sheep with intravaginal sponges. I. Effects of vaginal douche, supplementary steroids, time of insemination, numbers and dilution of spermatozoa. Austr J Agric Res 1970,21:767-781.
Willadsen SM.: Embryo transplantation in sheep. In the management and Diseases of Sheep. Eds. J.M.M. Cunningham, J.T. Stamp & W.B. Martin. The Commonwealth Agricultural Bureaux. Slough, U.K., 1979:69-85.
Evans G. and Armstrong DT.: Reduction of sperm transport in ewes by superovulation treatments. J Reprod Fert 1984, 70:47-53.
Hawk HW., Cooper BS. and Conley HH.: Inhibition of sperm transport and fertilization in superovulating ewes. Theriogenology 1987,28:139-153.
Killen ID. And Caffery GJ.: Uterine insemination of ewes with the aid of a laparoscope. Aust Vet J 1982,59:95.
Fieni F, Roques JM, Tainturier D, Bruyas JF. Utilisation du contrôle endoscopique pour Γ insemination intra-uterine chez les petit ruminants. Ree Med Vet 1992,168:295-302.
Armstrong DT and Evans G. Intrauterine insemination enhances fertility of frozen semen in superovulated ewes. J Reprod Fert 1984,71:89-94.
Jabbour H, Evans G. Fertility of superovulated ewes following intrauterine or oviductal insemination with fresh or frozenthawed semen. Reprod Fertil Dev 1991,3:1-7.
Maxwell WMC. Artificial insemination of ewes with frozenthawed semen at a synchronized oestrus. 2. Effect of dose of spermatozoa and site of intrauterine on fertility. Anim Reprod Sci 1986,10:309-316.
Kühholzer Β. Endoscopische embryotranfervefahren beim schaf. Diss med vet, Wien, 1996.
Kühholzer Β, Besenfelder U, Moller S, Reichenbach HD and Brem G. Laparoscopie insemination of seasonally anoestrous ewes by a simplified method under field conditions. Reprod Dom Anim 1997,32:309-312.
Scudamore CL, Robinson JJ, Aitken RP, Kennedy DJ, Ireland S and Robertson IS. Laparoscopy for insemination and embryo recovery in superovulated ewes at a commercial embryo transfer unit. Theriogenology 1991, 35:329-337.
Robinson JJ, Jacqueline M, Wallace M and Aitken RP. Fertilization and ovum recovery rates in superovulated ewes following cervical insemination or laparoscopic ontrauterine insemination at different tomes after progestagen withdrawal and in one or both uterine horns. J Reprod Fert 1989,87:771-782.
Mckelvey WAC, robinson JJ and Aitken RP. The evaluation of a laparoscopic insemination technique in ewes. Theriogenology 1985,24:519-535.
Meinecke Β and Meinecke-Tillmann S. Befruhtungsergebnisse bei superovulieten Schafen und Ziegen nach laparoskopisch kontrollierte transmural intrauteriner insemination. Tierärztl Prax 1986,14:35-41.
Scudamore CL, Robinson JJ, Aitken RP. The effect of timing of laparoscopic insemination in superovulated ewes, with or without sedation, on the recovery of embryos, their stage of development and subsequent viability. Theriogenology 1991,
:907-914.
Walker SK, Smith DH, Frensham A, Ashman RJ and Seamark RF. The use of synthetic gonadotropin releasing hormone treatment in the collection of sheep embryos. Theriogenology 1989, 31:741-752.
Σαμαρτζή Φ. Μελέτη της ανταπόκρισης των προβάτων φυλής Χίου στην πρόκληση πολλαπλής ωοθυλακιορηξίας μετά από χορήγηση PMSG και in vitro εκτίμησ της βιωσιμότητας των εμβρύων. Διδακτορική Διατριβή, Θεσσαλονίνη 1993.
Boscos C, Vainas Ε, Kouskoura Th, Samartzi F, Vafiadis D and Dellis S. Superovulatory response of Chios and Friesian ewes to two FSH-P dose levels. Reprod Dom Anim 1997, 32:195-198.
Meinecke-Tillmann S, Evars P, Meinecke Β and Gips Η. Problematik der PMSG-Supperovulationsbehandlung von Merinolandschafen im rahmen eines Embryotransferprogrammes: Beziehungen zwishen PMSG Plasmakonzentrationnen
und Superovulatioreaktion. Dt Tierärztl Wschr 1988, 95:137-192.
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