In-vitro efficacy of Arachis hypogaea (Peanut) peels extract against Haemonchus contortus

PDF (English)
DOI : https://doi.org/10.12681/jhvms.24082
A. TAHIR
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, 54000. Pakistan
R. AKHTAR
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, 54000. Pakistan
M. LATEEF
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, 54000. Pakistan
F. SATTAR
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, 54000. Pakistan
M.A. HAFEEZ
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, 54000. Pakistan
M.O. OMER
Department of Pharmacology, University of Veterinary and Animal Sciences, Lahore, 54000. Pakistan
B. ZAHID
Department of Pathology, College of Veterinary and Animal Sciences, Narowal, 51600. Pakistan
A. JAVED
Department of Pathology, University of Veterinary and Animal Sciences, Lahore, 54000. Pakistan
Résumé

Synthetic anthelmintics are becoming ineffective due to development of parasitic resistance. For this reason, traditional medicinal plants are being explored for their anthelmintic potential. The aim of this study was to evaluate the in-vitro anthelmintic activity of Arachis hypogaea L. (peanut) peels against Haemonchus contortus. To assess the anthelmintic effect of Arachis hypogaea L. on 3 life cycles of Haemonchus contortus, egg hatch assay (EHA), larval developmental assay (LDA) and adult motility assay (AMA) were conducted. In these tests, methanol and n-hexane extracts of the plant were used in three concentrations of 10, 15 and 20 mg/ml. Levamisole and PBS were used as positive and negative control groups respectively. Results of these tests showed that methanol extract of Arachis hypogaea L. had higher anthelmintic effect than that of n-hexane extract. Overall both extracts exhibited a significant (p<0.05) dose and a time dependent anthelmintic effect. At 20 mg/ml, methanol extract and n-hexane extract showed 87% and 80% egg hatching inhibition respectively. Methanol and n-hexane extracts at 20 mg/ml showed 83.3% and 76.6% larval mortality respectively. Adult motility test with both extracts showed maximum immobilization of worms after 6 hours of treatment at 20 mg/ml concentration. It is concluded that peels of Arachis hypogaea possess significant anthelmintic potential against nematodes. It may be suggested that the plant can be used further to investigate the in-vivo activity.

Article Details
  • Rubrique
  • Research Articles
Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Références
Adama K, Adama B, Tamboura H, Amadou T, Laya S (2009) In vitro anthelmintic effect of two medicinal plants (Anogeissus leiocarpus and Daniellia oliveri) on Haemonchus contortus, an abosomal nematode of sheep in Burkina Faso. Afr J Biotechnol, 8(18).
Al-Snafi AE (2014) Chemical constituents and pharmacological activities of Arachis hypogaea.–A review. Int J Pharm Res Schol, 3(1-1), pp.615-623.
Amin H, Ali T, Ahmad M, Zafar MI (2010) Gender and development: roles of rural women in livestock production in Pakistan. Pak. J. Agri. Sci. 2010 Jan 1;47(1):32-6.
Bilal MQ, Hameed A, Ahmad T (2009) Prevalence of gastrointestinal parasites in buffalo and cow calves in rural areas of Toba Tek Singh, Pakistan. J. Anim. Plant Sci. 2009;19(2):67-70.
Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA, Waller PJ (1992) World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol, 44(1-2),
pp.35-44.
Eysker M, Ploeger HW (2000) Value of present diagnostic methods for gastrointestinal nematode infections in ruminants. Parasitol, 120(7), pp.109-119.
Ferreira LE, Castro PM, Chagas AC, França SC, Beleboni RO (2013) In vitro anthelmintic activity of aqueous leaf extract of Annona muricata L.(Annonaceae) against Haemonchus contortus from sheep. Exp Par asitol, 134(3), pp.327-332.
Gadahi JA, Arshed MJ, Ali Q, Javaid SB, Shah SI (2009) Prevalence of gastrointestinal parasites of sheep and goat in and around Rawalpindi and Islamabad, Pakistan. Vet World, 2(2), p.51.
Githigia SM, Thamsborg SM, Maingi N, Munyua WK (2005) The epidemiology of gastrointestinal nematodes in Goats in the low potential areas of Thika District, Kenya. Bull Anim Health Prod Afr, 53(1),
pp.5-12.
Hounzangbe-Adote MS, Paolini V, Fouraste I, Moutairou K, Hoste H (2005) In vitro effects of four tropical plants on three life-cycle stages of the parasitic nematode, Haemonchus contortus. Res Vet Sci, 78(2), pp.155-160.
Iqbal Z, Lateef M, Khan MN, Jabbar A, Akhtar MS (2006) Anthelmintic activity of Swertia chirata against gastrointestinal nematodes of sheep. Fitoterapia. 2006 Sep 1;77(6):463-5.
Kamaraj C, Rahuman AA (2011) Efficacy of anthelmintic properties of medicinal plant extracts against Haemonchus contortus. Res Vet Sci, 91(3), pp.400-404.
Kaplan RM (2004) Drug resistance in nematodes of veterinary importance: a status report. Trends Parasitol, 20(10), pp.477-481.
Lewis WH (1977) Lewis. Medicinal botany plants affecting man’s health. Marie-Magdeleine C, Mahieu M, D’alexis S, Philibert L, Archimede H (2010) In vitro effects of Tabernaemontana citrifolia extracts on Haemonchus contortus. Res Vet Sci, 89(1), pp.88-92.
Mehlhorn H (2008) Encyclopedia of Parasitology. Springer-Verlag Berlin
Heidelberg. New York (eds). 2008.
Naumann HD, Armstrong SA, Lambert BD, Muir JP, Tedeschi LO, Kothmann MM (2014) Effect of molecular weight and concentration of legume condensed tannins on in vitro larval migration inhibition of Haemonchus contortus. Vet Parasitol, 199(1-2), pp.93-98.
Sargison ND (2012) Pharmaceutical treatments of gastrointestinal nematode infections of sheep—Future of anthelmintic drugs. Vet Parasitol, 189(1), pp.79-84.
Satyavati GV, Raina MK, Sharma M (1976) Medicinal Plants of India, vol. I, IndianCouncilof Med. Res., New Delhi, India. 1976:201-6.
Tiwari P, Kumar B, Kumar M, Kaur M, Debnath J, Sharma P (2011) Comparative anthelmintic activity of aqueous and ethanolic stem extract
of Tinospora cordifolia. Int J Drug Dev Res. 2011;3(1):70-83.
von Son-de Fernex E, Alonso-Díaz MA, Valles-de la Mora B, Capetillo-Leal CM (2012) In vitro anthelmintic activity of five tropical legumes on the exsheathment and motility of Haemonchus contortus infective larvae. Exp Parasitol, 131(4), pp.413-418.
Articles les plus lus par le même auteur ou la même autrice