The effect of paclitaxel (taxol) on the reproductive system and the semen parameters as well as on other organs of male rats.


The effect of the administration of paclitaxel on the male reproductive organs as well as on several semen parameters and other organs of Wis tar rats was studied. To 14 weeks old rats 12,4 mg paclitaxel/kg b.w.were administered i.p. once a week for 4 weeks. Six days after the last injection of paclitaxel ten animals, five (n=5) test (group Al) and five control animals (group A2) were sacrificed. Another ten animals, five (n=5) test (group Bl) and five (n=5) control animals (group B2) were sacrificed eleven weeks after the last injection of paclitaxel. The weights of the testes, the epididymis and the prostate gland of the test animals (group Al) were decreased as compared to the control animals (group A2). The number and the motility of the test animal spermatozoa were decreased, and many of them were abnormal. Histologically, the dividing cells and round spermatids were most affected. Leydig cells appeared degenerated. The epithelium of the ductucs epididymis was cuboidal and cell microvilli were absent. There was infiltration of neutrophils. The prostate gland was cystic with degeneration and desquamation of the epithelial cells. In the adrenals cellular degeneration and histiocytic infiltration were observed. In the spleen myeloid metaplasis and in the liver local allergic reaction around the interlobular bile ducts were observed. The weights of the testes, the epididymis and the prostate glands of the test animals (group Bl) were normal as compared with the control animals (group B2). The sperm motility was also normal but the number of spermatozoa was still decreased as compared with the control animals (group B2). Histologically, all examined organs were similar to those of the control animals.

Article Details
  • Section
  • Research Articles
Download data is not yet available.
Meistrich ML. Critical components of testicular function and sensitivity to disruption. Biol Reprod 1986,34:17-28
Dougherty RC, Whitaker MJ, Tang SY. Sperm density and toxic substances a potential key to environmental health hazards. In: McKinney JD (ed) Environmental health chemistry. Ann Arbor Sci, Ann Arbor, MI, 1981: 263-278
Brown T, Halvin K, Weiss G, Cagnola J., Koeller J., Kuhn J., Rizzo J., Craig J., Phillips J., von Hoff D. A phase I trial of Taxol given by a 6-hour intravenous infusion. J Clin Oncol 1991,9 (7): 1261-1267
Manfredi JJ, Horwitz SB. Taxol : an antimitotic agent with a new mechanism of action. Pharmac Ther 1984,25: 83-125
Schiff PB, Fant J, Horwitz SB. Promotion of microtubule assembly in vitro by taxol. Nature, 1979,227: 665-66
Kumar N. Taxol induces polymerization of purified tubulin. J Biol Chem 1981,256:1435-1441
Schiff PB, Horwitz SB. Taxol assembles tubulin in the absence of exogenous guanosine 5-triphosphate or microtubuleassociated proteins. Biochemistry 1981,20: 3242-3252
Rowinsky EK, Cazenave LA, Donehower RC. Taxol: a novel investigational antimicrotubule agent. J Natl Cancer Inst 1990,82 (15): 1247-1259
Neely MD, Boekelheide K. Sertoli cell processes have axoplasmic features: an ordered microtubule distribution and an abundant high molecular weight microtubule-associated protein (cytoplasmic dynein). J Cell Biol 1988,107 (5): 1767-1776
DoKhac L, Tanfin Z, Harbon S. Differential role of microtubules in the control of prostaglandin E 2 and betaadrenergic stimulation of cyclic AMP accumulation in the rat.
Biochem Pharmacol 1983,32(17): 2535-2541
Soederstroem KO, Roeyttae M. Short-time effects of taxol on the seminiferous epithelium of the rat. Cell Tissue Res 1, 1986,245:591-598
Rowinsky EK, Gilbert MR, Mc Guire WP, Noe D.A., Grochow L.B., Forastiere A.A., Ettinger D.S., Lubejho B.G., Clark B., Sarorius S.E., Cornblath DR,Hendrichs CB, and Donehower RC. Sequences of Taxol and Cisplatin: a phase I and pharmacologic study. J Clin Oncol 1991,9 (9): 1692-1703
Monsarrat B, Mariel E, Cros S, Gareo M, Guenard D, Gueritte- Voegeloin F, and Wright M. Taxol metabolismisolation and identification of three major metabolites of taxol in rat bile. Drug Metab Dispos 1990,18(6): 895-901
Ikegawa SJ, Hata K, Nkatomi H, Asaga M, Kaji S, Sugawara H, Uno I, Yoshihiro. Collaborative work to determine the optimal administration period and parameters to detect dug effects on male rat fertility.-Study on estratiol benzoate effects.J Toxicol Sci, 1995,20(3), 251-263
Lock LF, Soares ER. Increases in morphologically abnormal sperm in rats exposed to 60 Co irradiation. Environ Mutagen 1980,2:125-131
Hayes WA. Principles and Methods of Toxicology. Reproductive Toxicology. New York, Raven Press, 3rd Edit, 1994,962-966
Gangolli SD, Phillips JC. Assessing Chemical injury to the reproductive system. In: Anderson D, Conning DM (Eds) Experimental Toxicology, The basic issues, 2nd Edition,
Royal Society of Chemistry, 1993,376-394
Takayama S, Akaike M, Kawashima K, Takahashi M, Kurokawa Y. Studies on the optimal treatment period and parameters for detection of male fertility disorder in rats. Introductory summary. J Toxicol Sci, 1995,20:173-182
Imahie H, Adachi T, Nakagawa Y, Nagasaki T, Yamamura T, Hori M. Effects of adriamycin, an anticancer drug showing testicular toxictity, on fertility in male rats. J Toxicol Sci, 1995, 20 (3): 183-193
Mizoguchi K. Tsuno T, Hara H, Tanaka N, Igarashi S. Effects of a new platinum complex on male fertility in rats. J Toxicol Sci 1995,20(3): 207-216
Okada F, Niwa N, Hosokawa S, Kawaguchi T, Okuda Y, Matsubara Y, Yamatsu K, Igarashi T. Effects of repeated doses of compound E for 4 and 9 weeks on the male reproductive organs. J Toxicol Sci 1995,20(3): 217-227
Kinefelter GR, Laskey JW, Roberts NR, Slott V, Suarez J.D. Multiple effects of ethane dimathanesulfonate on the epididymis of adult rats. Toxicol Appi Pharmacol 1990,105:271-287
Benirschke K, Garner FM, Jones TC. Pathology of Laboratory Animals. Springer Verlag, New York, 1978, Vol 1:9
Most read articles by the same author(s)