Rabbit semen fertility after heterospermic insemination with spermatozoa of different capacitation time


P. YPSILANTIS (Π. ΥΨΗΛΑΝΤΗΣ)
Ph. SARATSIS (Φ. ΣΑΡΑΤΣΗΣ)
S. SAMOUILIDIS (Σ. ΣΑΜΟΥΗΛΙΔΗΣ)
Abstract

The influence of mixing heterogeneous sperm populations in rabbit semen fertility was studied. Eighty female rabbits which were divided in 4 groups (1,2,3 and 4) in = 20.) and 3 males of proven fertility were employed. The animals of group 1,2 and 3 were inseminated with semen from male A j, A2 and A3, respectively, while those of group 4 were inseminated with a mixture containing equal number of progressively motile spermatozoa from each of the above mentioned males ( A 1 + 2 + 3 ) (heterospermic insemination). Animals of each group were divided into 4 subgroups according to the insemination time (15,10, 5 and 0 hours prior the expected ovulation). In each group, differences were observed (P<0.05) between subgroups at the percentage of animals that delivered, indicating differences between males at the time and the duration of sperm capacitation in vivo. Based on the percentage of the animals that delivered and the litter size, semen fertility was improved after the application of heterospermic insemination, at all insemination times. This improvement was attributed to the extension of the time during which capacitated spermatozoa were present in the female genital tract due to the mixture of heterogeneous sperm populations of different capacitation time.

Article Details
  • Sezione
  • Research Articles
Downloads
I dati di download non sono ancora disponibili.
Riferimenti bibliografici
Eddy EM. The spermatozoon. In: Knobil E, Neill I et al (Eds) The physiology of reproduction. Raven Press, New York, 1988:27-68.
Yanagimachi R. Mammalian fertilization. In: Knobil E, Neill I et al (Eds). The physiology of reproduction. Raven Press, New York, 1988:135-185.
Yanagimachi R. Mechanisms of fertilization in mammals. In: Mastroianni L and Biggers JB (Eds). Fertilization and Embryonic Development in vitro. Plemun Press, New York, 1981:81-182.
Bedford JM. Significance of the need for sperm capacitation before fertilization in eutherian mammals. Biol Reprod 1983,28:108-120.
Cuasnicu PS, Bedford JM. The effect of moderate epididymal aging on the kinetics of the acrosome reaction and fertizing ability of hamster spermatoza. Biol Reprod 1989,40:1067-1073.
Harrison RA. Capacitation mechanisms and the role of capacitation as seen in eutherian mammals. Reprod Fertil Devl996,8(4):581-594.
Parrish JJ, Foote RH. Fertility differences among male rabbits determined by heterospermic insemination of fluorochrome-labeled spermatozoa. Biol Reprod 1985,33:940-949.
Chen Y, Li M, Simkin E, Yang X, Foote RH. Fertility of fresh and frozen rabbit semen inseminated at different times is indicative of male differences in capacitation time. Biol Reprod 1989,41:848-853.
Berger T. Proportion of males with lower fertility spermatozoa estimated from heterospermic insemination. Theriogenology 1995,43:769-775.
Chen Y, Yang X, Foote RH. Timed breeding of rabbits with fresh and frozen - thawed semen and evidence of acrosome alteration following freezing and thawing. Anim Reprod Sci 1989,18:35-41.
ΙΙ.Σφαιρόπουλος Α. Κονικλοτροφία. Υπηρεσία Δημοσιευμάτων ΑΠΘ, Θεσσαλονίκη, 1991:55-73.
Castellini C. Σύγχρονες τεχνικές στην αναπαραγωγή των κουνελιών και δυνατότητες εφαρμογής τους. Πρακτικά 1ης Ελληνο ιταλική ς Επιστημονικής Διημερίδας. 3-4 Φεβρουαρίου, θεσσαλονίκη, 1995:19-28.
Hanada Α, Nagase Η. Cryopreservative effects of some amides on rabbit spermatozoa. J Reprod Fertil 1980; 60:247-252.
Kim CK, Im KS, Zheng X, Foote RH. In vitro capacitation and fertilizing ability of ejaculated rabbit sperm treated with lysophosphatidylchloline. Gam Res 1989,22:131-141.
Young RJ, Bodt BA, Heitkamp DH. Action of metallic ions on the precocious development by rabbit sperm of motion patterns that are characteristic of hyperactivated motility. Mol Reprod Dev 1995,41(2):239-248.
Yanagimachi R, Usui N. Calcium dependence of the acrosome reaction and activation of guinea pig spermatozoa. Exp Cell Res 1974,89:161-171.
Talbot P, Franklin LE. Morphology and kinetics of the hamster sperm acrosome reaction. J Exp Zool 1976,198:163-176.
Ward CR, Storey BT. Determination of the time course of capacitation in mouse spermatozoa using a chlorotetracycline fluoresence assay. Dev Biol 1984,104:287-196.
0verstreet JW, Cooper GW. The time and location of the acrosome reaction during sperm transport in the female rabbit. J Exp Zool 1979,209:97-104.
Cummins JM. Hyperactivated motility patterns of ram spermatozoa recoverd from oviducts of mated ewes. Gamete Res 1982, 6:53-63.
Cummins JM, Yamagimachi R. Sperm/egg ratios and the state of the acrosome reaction during in vivo fertillization in the hamster. Gamete Res 1982,5:239-256.
Bedford JM. Sperm capacitation and fertilization in mammals. Biol Reprod 1970 (Suppl 2):128-158.
Beatty RA, Bennet GH, Hall JG, Hancock JL, Stewart DL. An experiment with heterospermic insemination in cattle. J Reprod Fertil 1969,19:491-502.
O'Reilly PJ, Graves CN, Dziuk PJ. Heterospermic insemination of rabbit semen as a means of evaluating techniques of semen handling. J Reprod Fertil 1972, 29:49-56.
Stewart DL, Spooner RL, Bennet GH, Beatty RA, Hancock JL. A second experiment with heterospermic insemination in cattle. J Reprod Fertil 1974,36:107-116.
Overstreet JW, Adams CR. Mechanisms of selective fertilization in the rabbit: sperm transport and viability. J Reprod Fertil 1971,26:219-231.
Ficher Β, Adams CE. Fertilization following mixed insemination with "cervix selected" and "unselected" spermatozoa in the rabbit. J Reprod Fertil 1981,62:337-343.
Puoi leggere altri articoli dello stesso autore/i