Freezing of rabbit semen after the addition of glycerol and dimethylsulfoxide in the extender
Abstract
In this study, semen from 10 rabbits was frozen after it was diluted in two different extenders, A and B. Extender A consisted of Tris-buffer in which 20% of egg yolk and 3% of glycerol were added, while extender Β had the same composition, plus 3% of dimethylsulfoxide (DMSO). The semen of the buck exhibiting the best post-thaw motility in the two extenders, was used for the insemination of two corresponding groups (I and II) of 20 does each. The average sperm motility of fresh semen (77%) was significantly reduced (P<0.05) after it was frozen in the extender A (47%) and Β (54%). The post-thaw sperm motility in extender Β was significantly higher (P<0.05) than that in extender A. The percentage of the animals that gave birth in group II (60%) was increased compared to that of the animals in group I (50%), but not significantly (P>0.05). Also, no significant difference (P>0.05) was observed between the average litter size of group I (6,8±1,7) and group II (7,0±1,9). Thereafter, it is concluded that freezing rabbit semen in an extender that contains a combination of the cryoprotective agents glycerol and DMSO, in the proportion of 3% and 3%, respectively, results in a significant improvement of post-thaw sperm motility, while it doesn't affect significantly its fertility value neither the size of the litters.
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SAMOUILIDIS (Σ. ΣΑΜΟΥΗΛΙΔΗΣ) S., SAOULIDIS (Κ. ΣΑΟΥΛΙΔΗΣ) K., FOUKOS (Α. ΦΟΥΚΟΣ) A., YPSILANTIS (Π. ΥΨΗΛΑΝΤΗΣ) P., DEMERTZIS (Α. ΔΕΜΕΡΤΖΗΣ) A., & SBIRAKI (Α.Π. ΣΜΠΙΡΑΚΗ) A. P. (2018). Freezing of rabbit semen after the addition of glycerol and dimethylsulfoxide in the extender. Journal of the Hellenic Veterinary Medical Society, 52(4), 299–302. https://doi.org/10.12681/jhvms.15461
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- Vol. 52 No. 4 (2001)
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- Research Articles
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