Questionnaire-based survey on canine leishmaniosis (Leishmania infantum) in Greece


Опубликован: Νοε 21, 2017
M. N. SARIDOMICHELAKIS (Μ.Ν. ΣΑΡΙΔΟΜΙΧΕΛΑΚΗΣ)
A. F. KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ)
P. BOURDEAU
Аннотация

On November 2006, a total of 1,144 questionnaires related to various aspects of canine leishmaniosis (CanL) were mailed to Greek companion animal practitioners. The overall return rate was 17.6% (201/1,144) and did not differ among the nine geographical areas of the country where their practices were located. The global minimum, average and maximum frequency of CanL diagnosis, on a year-round basis, were 0.98%, 3.30% and 7.46%, respectively; these figures were lower in Crete, followed by Aegian islands and then by Macedonia, compared to Péloponnèse, Central Greece (including Evoia) and Thessaly. Most of the veterinarians (70.4%) were practicing in endemic areas of the disease with the number of new cases and cases under treatment reported to remain stable over the past years. The most common clinical manifestations of CanL reported were the progressive loss of body weight, peripheral lymphadenomegaly, anemia and exoliative dermatitis with or without alopecia; concurrent diseases, especially ehrlichiosis and dirofilariosis, were also commonly witnessed. The abnormal results of non-specific laboratory examinations, such as haematology, serum biochemistry and urinalysis raised the suspicion of CanL that was subsequently confirmed mainly with serology (in-office rapid screening tests, indirect immunofluorescence, ELISA) and less often with lymph node smear microscopy and PCR. Allopurinol and pentavalent antimonials, usually in combination, and less frequently metronidazole, ketoconazole, amphotericin B, fluoroquinolones or aminosidine were employed as antileishmanial medication in the cases undergone treatment. Pentavalent antimonials were associated with a higher frequency of side effects, followed by amphotericin B, whereas allopurinol was better tolerated. Side effects necessitating the discontinuation of treatment were accounted in approximately 14% of the treated dogs. The discontinuation of treatment was mainly based on serology, although additionalcriteria, such as the clinical improvement or cure, and the normalization of routine laboratory results, were also taken into account. The majority of practitioners repeated the treatment in the advent of a clinical relapse of their cases. Euthanasia was initially suggested by a few veterinarians, but eventually it was necessary in approximately 10% of the treated dogs. The widespread use of insecticides and/or insect repellents, with or without taking additional preventative measures, such as keeping the dog indoors during the night and applying a fine mesh on the screens, was also recorded. Finally, almost all the practitioners made dog owners aware of the public health issues associated with CanL; however, in most of the cases such information did not deter the owners from accepting treatment of their dogs.

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Acedo Sanchez C, Martin Sanchez J, Vélez Bernal ID, Sanchis Marin MC, Louassini M, Maldonado JA, Morulas Marquez F (1996) Leishmaniasis eco-epidemiology in the Alpujarra region (Granada Province, southern Spain). International Journal for Parasitology, 26:303-310.
Adamama-Moraitou KK, Saridomichelakis MN, Polizopoulou Z, Kritsepi M, Tsompanakou A, Koutinas AF (2005) Short-term exogenous glucocorticosteroidal effect on iron and copper status in canine leishmaniasis (Leishmania infantum). Canadian Journal of Veterinary Research, 69:287-292.
Andrande HM, Reis AB, Santos SL, Volpini AC, Marques MJ, Romanha AJ (2006) Use of PCR-RFLP to identify Leishmania species in naturally-infected dogs. Veterinary Parasitology, 140:231-238.
Argyriadis D, Litke Ο (1991) Epizootiological study of dog leishmaniasis in Central and Eastern Macedonia and in Thessaly. Bulletin of the Hellenic Veterinary Medical Society, 42:30-34.
Ashford RW, Snowden KF (2000) The leishmaniases. In: Dogs, Zoonoses and Public Health. CABI Publishing, Oxon and New York, pp 132-140.
Athanasiou LV, 2004. Contribution to the study of epidemiology, diagnosis and treatment of canine leishmaniosis. Doctoral thesis. Aristotles University of Thessaloniki, Thessaloniki, Greece.
Barbosa-De-Deus R, Dos Mares-Guia ML, Nunes AZ, Costa KM, Junqueira RG, Mayrink W, Genaro O, Tavares CA (2002) Leishmania major-like antigen for specific and sensitive serodiagnosis of human and canine visceral leishmaniasis. Clinical and Diagnostic Laboratory Immunology, 9:1361-1366.
Berrahal F, Mary C, Roze M, Berenger A, Escoffier K, Lamouroux D, Dunan S (1996) Canine leishmaniasis: identification of asymptomatic carriers by polymerase chain reaction and immunoblotting. American Journal of Tropical Medicine and Hygiene, 55:273-27.
Blavier A, Keroack S, Denerolle P, Goy-Thollot I, Chabanne L, Cadore JL, Bourdoiseau G (2001) Atypical forms of canine leishmaniosis. The Veterinary Journal, 162:108-120.
Bourdeau P, Mallet M, Marchand A (2005) Clinical aspects in canine leishmaniosis (Leishmania infantum): results from a national survey in France. In: Proceedings of the 20th Annual Congress of the E.S.V.D.-E.C.V.D., (Chalkidiki, Greece), p 172.
Boutsini SG, Patarroyo M J (2001) Canine leishmaniosis in Attiki between 1995-2000. In: Proceedings of the 6th Hellenic Congress in Small Animal Veterinary Medicine, (Athens, Greece), pp 117-120.
Cabrai M, O'Grady JE, Gomes S, Sousa JC, Thompson H, Alexander J (1998) The immunology of canine leishmaniosis: strong evidence for a developing disease spectrum from asymptomatic dogs. Veterinary Parasitology, 76:173-180.
Cabrai M, O'Grady J, Alexander J (1992) Demonstration of Leishmania specific cell mediated and humoral immunity in asymptomatic dogs. Parasite Immunology, 14:531-539.
Cardoso L, Neto F, Sousa JC, Rodrigues M, Cabrai M (1998) Use of a leishmanin skin test in the detection of canine Leishmaniaspecific cellular immunity. Veterinary Parasitology, 79:213-220.
Carrió J, Portus M (2002) In vitro susceptibility to pentavalent antimony in Leishmania infantum strains is not modified during in vitro or in vivo passages but is modified after host treatment with meglumine antimoniate. BMC Pharmacology, 2:11.
Cenini P, Berhe N, Hailu A, McGinnes K, Frommel D (1993) Mononuclear cell subpopulations and cytokine levels in human visceral leishmaniasis before and after chemotherapy. The Journal of Infectious Diseases, 168:986-993.
Ciaramella Ρ, Corona M (2003) Canine leishmaniasis: therapeutic aspects. Compendium of Continuing Education for the Practicing Veterinarian, 25:370-375.
Ciaramella Ρ, Oliva G, DeLuna R, Gradoni L, Ambrosio R, Cortese L, Scalone A, Persechino A (1997) A retrospective clinical study of canine leishmaniasis in 150 dogs naturally infected by Leishmania infantum. The Veterinary Record, 141:539-543.
Cortese L, Pelagalli A, Piantedosi D, Mastellone V, Loria AD, Lombardi Ρ, Ciaramella P, Avallone L (2008) The effects of prednisone on haemostasis in leishmaniotic dogs treated with meglumine antimoniate and allopurinol. The Veterinary Journal, 177:405-410.
Cringoli G, Rinaldi L, Capuano F, Baldi L, Veneziano V, Capelli G (2002) Serological survey of Neospora caninum and Leishmania infantum co-infection in dogs. Veterinary Pathology, 106:307-313.
da Costa-Val AP, Cavalcanti RR, de Figueiredo Gontijo N, Michalick MS, Alexander B, Williams P, Melo MN (2007) Canine visceral leishmaniasis: relationships between clinical status, humoral immune response, haematology and Lutzomyia (Lutzomyia) longipalpis infectivity. The Veterinary Journal, 174:636-643.
da Silva ES, van der Meide WF, Schoone GJ, Gontijo CMF, Schallig HDFH, Brazil RP (2006) Diagnosis of canine leishmaniasis in the endemic area of Belo Horizonte, Minas Gerais, Brazil by parasite, antibody and DNA detection assays. Veterinary Research Communications, 30:637-643.
Dantas-Torres F (2007) The role of dogs as reservoirs of Leishmania parasites, with emphasis on Leishmania (Leishmania) infantum and Leishmania (Viannia) braziliensis. Veterinary Parasitology, 149:139-146.
de Fatima Madeira M, de Ο Schubach A, Schubach TM, Pereira SA, Figueiredo FB, Baptista C, Leal CA, Melo CX, Confort EM, Marzochi MC (2006) Post mortem parasitological evaluation of dogs seroreactive for Leishmania from Rio de Janeiro, Brazil. Veterinary Parasitology, 138:366-370.
Dereure J, Pratlong F, Dedet JP (1999) Geographical distribution and the identification of parasites causing canine leishmaniasis in the Mediterranean basin. In: Proceedings of the International Canine Leishmaniasis Forum, (Barcelona, Spain), pp 18-25.
Dietze R, Barros GB, Teixeira L, Harris J, Michelson K, Falqueto A,Corey R (1997) Effect of eliminating seropositive canines on the transmission of visceral leishmaniasis in Brazil. Clinical Infectious Diseases, 25:1240-1242.
Dietze R, Falqueto A, Valli LC, Rodriques TP, Boulos M, Corey R (1995) Diagnosis of canine visceral leishmaniasis with a dotenzyme-linked immunosorbent assay. American Journal of Tropical Medicine and Hygiene, 53:40-42.
Diniz SA, Melo MS, Borges AM, Bueno R, Reis BP, Tafuri WL, Nascimento EF, Santos RL (2005) Genital lesions associated with visceral leishmaniasis and shedding of Leishmania sp. in the semen of naturally infected dogs. Veterinary Pathology, 42:650-658.
Dos-Santos WL, Jesus EE, Paranhos-Silva M, Pereira AM, Santos JC, Baleeiro CO, Nascimento EG, Moreira ED, Oliveira GG, Pontesde-Carvalho LC (2008) Associations among immunological, parasitological and clinical parameters in canine visceral leishmaniasis: Emaciation, spleen parasitism, specific antibodies and leishmanin skin test reaction. Veterinary Immunology and Immunopathology, 123:251-259.
Dye C, Vidor E, Dereure J (1993) Serological diagnosis of leishmaniasis: on detecting infection as well as disease. Epidemiology and Infection, 103:647-656.
Fernandez-Bellon H, Solano-Gallego L, Rodriguez A, Rutten VP, Hoek A, Ramis A, Alberola J, Ferrer L (2005) Comparison of three assays for the evaluation of specific cellular immunity to Leishmania infantum in dogs. Veterinary Immunology and Immunopathology, 107:163-169.
Ferreira Ede C, de Lana M, Carneiro M, Reis AB, Paes DV, da Silva ES, Schallig H, Gontijo CM (2007) Comparison of serological assays for the diagnosis of canine visceral leishmaniasis in animals presenting different clinical manifestations. Veterinary Parasitology, 146:235-241.
Ferrer L (1992) Leishmaniasis. In: Current Veterinary Therapy XISmall Animal Practice. XL W.B. Saunders, Philadelphia, pp 266-270.
Ferrer L (2004) Simultaneous presentation of leishmaniosis and other infectious diseases: clinical approach and mechanisms. In: Proceedings of the International Congress on Canine Leishmaniasis, (Naples, Italy), pp 37-38.
Foglia Manzillo V, Oliva G, Pagano A, Manna L, Maroli M, Gradoni L (2006) Deltamethrin-impregnated collars for the control of canine leishmaniasis: Evaluation of the protective effect and influence on the clinical outcome of Leishmania infection in kennelled stray dogs. Veterinary Parasitology, 142:142-145.
Gaskin AA, Schantz P, Jackson J, Birkenheuer A, Tomlinson L, Gramiccia M, Levy M, Steuer F, Kollmar E, Hegarty BC, Ahn A, Breitschwerdt EB (2002) Visceral leishmaniasis in a New York foxhound kennel. Journal of Veterinary Internal Medicine, 16:34-44.
Gavgani AS, Hodjati MH, Mohite H, Davies CR (2002) Effect of insecticide-impregnated dog collars on incidence of zoonotic visceral leishmaniasis in Iranian children: a matched-cluster randomised trial. The Lancet, 360:374-379.
Ginel PJ, Lucena R, Lopez R, Molleda JM (1998) Use of allopurinol for maintenance of remission in dogs with leishmaniasis. Journal of Small Animal Practice, 39:271-274.
Gradoni L (1999) Epizootiology of canine leishmaniasis in southern Europe. In: Proceedings of the International Canine Leishmaniasis Forum, (Barcelona, Spain), pp 32-39.
Gradoni L (2002) The diagnosis of canine leishmaniasis. In: Proceedings of the 2nd International Canine Leishmaniasis Forum, (Sevilla, Spain), pp 7-14.
Gradoni L, Foglia Manzillo V, Pagano A, Piantedosi D, De Luna R, Gramiccia M, Scalone A, Di Muccio T, Oliva G (2005) Failure of a multi-subunit recombinant leishmanial vaccine (MML) to protect dogs from Leishmania infantum infection and to prevent disease progression in infected animals. Vaccine, 23:5245-5251.
Iniesta L, Fern_ndez-Barredo S, Bulle Β, G_mez MT, Piarroux R, GJlego M, Alunda JM, Port_s M (2002) Diagnostic techniques to detect cryptic leishmaniasis in dogs. Clinical and Diagnostic Laboratory Immunology, 9:1137-1141.
Killick-Kendrick R, Killick-Kendrick M, Focheux C, Dereure J, Puech MP, Cadiergues MC (1997) Protection of dogs from bites of phlebotomine sandflies by deltamethrin collars for control of canine leishmaniasis. Medical and Veterinary Entomology, 11:105-
Knechtli R, Jenni L (1990) Experimental transmission of Leishmania infantum by the bite of Phlebotomus perniciosus from Switzerland. Acta Tropica, 47:213-216.
Kontos VI, 1986. A contribution to the study of canine leishmaniasis, A clinical, serological and experimental investigation. Doctoral thesis. Aristotelian University of Thessaloniki, Thessaloniki, Greece.
Kontos VJ, Koutinas AF (1993) Old World canine leishmaniasis. Compendium of Continuing Education for the Practicing Veterinarian, 15:949-960.
Koutinas AF, Polizopoulou ZS, Saridomichelakis MN, Argyriadis D, Fytianou A, Plevraki KG (1999) Clinical considerations on canine visceral leishmaniasis (CVL) in Greece: a retrospective study of158 spontaneous cases. Journal of the American Animal HospitalAssociation, 35:376-383.
Koutinas AF, Saridomichelakis MN, Mylonakis ME, Leontides L, Polizopoulou Z, Billinis C, Argyriadis D, Diakou N, Papadopoulos Ο (2001) A randomised, blinded, placebo-controlled clinical trial with allopurinol in canine leishmaniosis. Veterinary Parasitology, 98:247-261.
Koutinas AF, Scott DW, Kontos V, Lekkas S (1992) Skin lesions in canine leishmaniasis (Kala-Azar): a clinical and histopathological study on 22 spontaneous cases in Greece. Veterinary Dermatology, 3:121-130.
Koutinas CK, 2006. Efficacy and safety of amphotericin Β (lipid emulsion) in canine leishmaniosis (Leishmania infantum) and its effect on renal function, along with or without enalapril. Doctoral thesis. Aristotles University of Thessaloniki, Thessaloniki, Greece.
Leandro C, Santos-Gomes GM, Campino L, Româo Ρ, Cortes S, Rolâo Ν, Gomes-Pereira S, Rica Capela MJ, Abranches Ρ (2001) Cell mediated immunity and specific IgGl and IgG2 antibody response in natural and experimental canine leishmaniosis. Veterinary Immunology and Immunopathology, 79:273-284.
Leontides LS, Saridomichelakis MN, Billinis C, Kontos V, Koutinas AF, Galatos AD, Mylonakis ME (2002) A cross-sectional study of Leishmania spp. infection in clinically healthy dogs with polymerase chain reaction and serology in Greece. Veterinary Parasitology, 109:19-27.
Maroli M (2004) Leishmap: the network for monitoring the spreading of canine leishmaniasis and phlebotomine vectors in Northern Italy. In: Proceedings of the International Congress on Canine Leishmaniasis, (Naples, Italy), pp 109-112.
Maroli M, Mizzon V, Siragusa C, D'Oorazi A, Gradoni L (2001) Evidence for an impact on the incidence of canine leishmaniasis by the mass use of deltamethrin-impregnated dog collars in southern Italy. Medical and Veterinary Entomology, 15:358-363.
Miranda S, Roura X, Picado A, Ferrer L, Ramis A (2008) Characterization of sex, age, and breed for a population of canine leishmaniosis diseased dogs. Research in Veterinary Science, 85:35-38.
Mirò G, Cardoso L, Pennisi MG, Oliva G, Baneth G (2008) Canine leishmaniosis - new concepts and insights on an expanding zoonosis: part two. Trends in Parasitology, 24:371-377.
Moreira ED, Mendes de Souza VM, Sreenivasan M, Nascimento EG, Pontes de Carvalho L (2004) Assessment of an optimized dogculling program in the dynamics of canine Leishmania transmission. Veterinary Parasitology, 122:245-252.
Moreno J, Alvar J (2002) Canine leishmaniasis: epidemiological risk and the experimental model. Trends in Parasitology, 18:399-405.
Mozos E, Perez J, Day M J, Lucena R, Ginel Ρ J (1999) Leishmaniosis and generalized demodicosis in three dogs: a clinicopathological and immunohistochemical study. Journal of Comparative Pathology, 120:257-268.
Nejjar R, Lemrani M, Boucedda L, Amarouch H, Benslimane A (2000) Variation in antibody titres against Leishmania infantum in naturally infected dogs in northern Morocco. Revue de Médecine Vétérinaire, 151:841-846.
Noli C, Auxilia ST (2005) Treatment of canine Old World visceral leishmaniasis: a systematic review. Veterinary Dermatology, 16:213-232.
Oliva G, Gradoni L, Cortese L, Orsini S, Ciaramella Ρ, Scalone A, de Luna R, Persechino A (1998) Comparative efficacy of meglumine antimoniate and aminosidine sulphate, alone or in combination, in canine leishmaniasis. Annals of Tropical Medicine and Parasitology, 92:165-171.
Otranto D, Paradies Ρ, Lia RP, Latrofa MS, Testini G, Cantacessi C, Mencke N, Galli G, Capelli G, Stanneck D (2007) Efficacy of a combination of 10% imidacloprid/50% permethrin for the prevention of leishmaniasis in kennelled dogs in an endemic area. Veterinary Parasitology, 144:270-278.
Palatnik-de-Sousa CB, Silva-Antunes I, Morgado Ade A, Menz I, Palatnik M, Lavor C (2009) Decrease of the incidence of human and canine visceral leishmaniasis after dog vaccination with Leishmune® in Brazilian endemic areas. Vaccine, 27:3505-3512.
Papadogiannakis EI, Koutinas AF, Saridomichelakis MN, Vlemmas J, Lekkas S, Karameris A, Fytianou A (2005) Cellular immunophenotyping of exfoliative dermatitis in canine leishmaniosis (Leishmania infantum). Veterinary Immunology and Immunopathology, 104:227-237.
Papierok GM, Holtzmuller P, Hugnet C, Lemesre JL (2002) New drug and new methods of investigation for the therapy of canine leishmaniasis. In: Proceedings of the 18th E.S.V.D.-E.C.V.D. Annual Congress, (Nice, France), ρ 210.
Petanides TA, Koutinas AF, Mylonakis ME, Day MJ, Saridomichelakis MN, Leontides LS, Mischke R, Diniz P, Breitschwerdt EB, Kritsepi M, Garipidou VA, Koutinas CK, Lekkas S (2008) Factors associated with the occurrence of epistaxis in natural canine leishmaniasis (Leishmania infantum). Journal of Veterinary Internal Medicine, 22:866-872.
Pinelli E, Rutten VP, Bruysters M, Moore PF, Ruitenberg EJ (1999) Compensation for decreased expression of B7 molecules on Leishmania infantum-infected canine macrophages results in restoration of parasite-specific T-cell proliferation and gamma interferon production. Infection and Immunity, 67:237-243.
Plevraki K, Koutinas AF, Kaldrymidou H, Roumpies N, Papazoglou LG, Saridomichelakis MN, Sawas I, Leondides L (2006) Effects of allopurinol treatment on the progression of chronic nephritis in canine leishmaniosis (Leishmania infantum). Journal of Veterinary Internal Medicine, 20:228-233.
Plumb D (1995) Levamizole. In: Plumb's Veterinary Drug Handbook, pp 356-360.
Poot J, Rogers ME, Bates PA, Vermeulen A (2005) Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs. Veterinary Parasitology, 130:41-53.
Pozio E, Gradoni L, Bettini S, Gramiccia M (1981) Leishmaniasis in Tuscany (Italy): VI. Canine leishmaniasis in the focus of Monte Argentario (Grosseto). Acta Tropica, 38:383-393.
Quinnell RJ, Courtenay O, Garcez L, Dye C (1997) The epidemiology of canine leishmaniasis: transmission rates estimated from a cohort study in Amazonian Brazil. Parasitology, 115 143-156.
Rallis T, Day MJ, Saridomichelakis MN, Adamama-Moraitou KK, Papazoglou L, Fytianou A, Koutinas AF (2005) Chronic hepatitis associated with canine leishmaniosis (Leishmania infantum): a clinicopathological study of 26 cases. Journal of Comparative
Pathology, 132:145-152.
Reis AB, Teixeira-Carvalho A, Vale AM, Marques MJ, Giunchetti RC, Mayrink W, Liboreiro Guerra L, Andrade R, Correa-Oliveira R, Martins-Filho OA (2006) Isotype patterns of immunoglobulins: hallmarks for clinical status and tissue parasite density in Brazilian dogs naturally infected by Leishmania (Leishmania) chagasi. Veterinary Immunology and Immunopathology, 112:102-116.
Reithinger R, Lambson BE, Barker DC, Davies CR (2000) Use of PCR to detect Leishmania (Viannia) spp. in dog blood and bone marrow. Journal of Clinical Microbiology, 38:748-751.
Reithinger R, Teodoro U, Davies CR (2001) Topical insecticide treatments to protect dogs from sand fly vectors of leishmaniasis. Emerging Infectious Diseases, 7:872-876.
Rougier S, Vouldoukis I, Fournel S, Pérès S, Woehrlé F (2008) Efficacy of different treatment regimens of marbofloxacin in canine visceral leishmaniosis: a pilot study. Veterinary Parasitology, 153:244-254.
Roze M (2004) Canine leishmaniasis epidemiological aspects in Southern France. In: Proceedings of the International Congress on Canine Leishmaniasis, (Naples, Italy), pp 71-72.
Saridomichelakis MN, Mylonakis ME, Leontides LS, Billinis C, Koutinas AF, Galatos AD, Gouletsou P, Diakou A, Kontos VI (2005a) Periodic administration of allopurinol is not effective for the prevention of canine leishmaniosis (Leishmania infantum) in the endemic areas. Veterinary Parasitology, 130:199-205.
Saridomichelakis MN, Mylonakis ME, Leontides LS, Koutinas AF, Billinis C, Kontos VI (2005b) Evaluation of lymph node and bone marrow cytology in the diagnosis of canine leishmaniasis (Leishmania infantum) in symptomatic and asymptomatic dogs. American Journal of Tropical Medicine and Hygiene, 73:82-86.
Scalone A, De Luna R, Oliva G, Baldi L, Satta G, Vesco G, Mignone W, Turilli C, Mondesire RR, Simpson D, Donoghue AR, Frank GR, Gradoni L (2002) Evaluation of the Leishmania recombinant K39 antigen as a diagnostic marker for canine leishmaniasis and validation of a standardized enzyme-linked immunosorbent assay. Veterinary Parasitology, 104:275-285.
Sideris V, Karagoimi Ε, Papadopoulou G, Garifallou A, Dotsika E (1996) Canine visceral leishmaniasis in the great Athens area, Greece. Parasite, 3:125-130.
Sideris V, Papadopoulou G, Dotsika E, Karagouni E (1999) Asymptomatic canine leishmaniasis in Greater Athens area, Greece. European Journal of Epidemiology, 15:271-276.
Slappendel RJ, Ferrer L (1998) Leishmaniasis. In: Clinical Microbiology and Infectious Diseases of the Dog and Cat. 2nd ed, W.B. Saunders, Philadelphia, pp 450-458.
Slappendel RJ, Teske E (1999) A review of canine leishmaniasis presenting outside endemic areas. In: Proceedings of the International Canine Leishmaniasis Forum, (Barcelona, Spain), pp 54-59.
Solano-Gallego L, Llull J, Ramos G, Riera C, Arboix M, Alberala J, Ferrer L (2000) The Ibizian hound presents predominantly cellular immune response against natural Leishmania infection Veterinary Parasitology, 90:37-45.
Trees A, Shaw S (1999) Imported diseases in small animals. In Practice, 21:482-491.
Vamvakidis CD, Koutinas AF, Kanakoudis G, Georgiadis G, Saridomichelakis M (2000) Masticatory and skeletal muscle myositis in canine leishmaniasis (Leishmania infantum). The Veterinary Record, 146:698-703.
Vercammen F, Berkvens D, Le Ray D, Jacquet D, Vervoort Τ (1997) Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests. The Veterinary Record, 141:328-330.
Zaffaroni E, Rubaudo L, Lanfranchi P, Mignone W (1999) Epidemiological patterns of canine leishmaniosis in Western Liguria (Italy). Veterinary Parasitology, 81:11-19.
Zatelli A, Borgarelli M, Santilli R, Bonfanti U, Nigrisoli E, Zanatta R, Tarducci A, Guarraci A (2003) Glomerular lesions in dogs infected with Leishmania organisms. American Journal of Veterinary Research, 64:558-561.
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